中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (8): 1332-1337.doi: 10.4103/1673-5374.162769

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

微囊化嗅鞘细胞移植修复损伤坐骨神经：抑痛效果优于嗅鞘细胞移植

  

  • 收稿日期:2015-06-08 出版日期:2015-08-24 发布日期:2015-08-24
  • 基金资助:

    国家自然科学基金（81260190）；江西省自然科学基金项目（20132BAB205023）；江西省教育厅科学技术研究项目（GJJ13159）；江西卫生厅科技项目（20132019）

Microencapsulation improves inhibitory effects of transplanted olfactory ensheathing cells on pain after sciatic nerve injury

Hao Zhao1, 2, Bao-lin Yang1, Zeng-xu Liu1, Qing Yu3, Wen-jun Zhang2, Keng Yuan4, Hui-hong Zeng5, Gao-chun Zhu1, De-ming Liu1, Qing Li2   

  1. 1 Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China
    2 Medical Department, Graduate School, Nanchang University, Nanchang, Jiangxi Province, China
    3 Library, Nanchang University, Nanchang, Jiangxi Province, China
    4 Medical Office, Nanchang University, Nanchang, Jiangxi Province, China
    5 Department of Histology and Embryology, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China
  • Received:2015-06-08 Online:2015-08-24 Published:2015-08-24
  • Contact: Zeng-xu Liu, M.D., 1965349157@qq.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81260190; the Natural Science Foundation of Jiangxi Province of China, No. 20132BAB205023; a grant from the Science and Technology Research Program of Department of Education of Jiangxi Province in China, No. GJJ13159; a grant from the Science and Technology Program of Department of Health of Jiangxi Province, No. 20132019.

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摘要：

课题组前期的研究发现嗅球组织移植能抑制P2X2/3受体介导的神经病理痛, 但嗅球组织内含多种细胞及成分，纯化的移植嗅鞘细胞如何作用尚不明确。为此，本次实验分别将嗅鞘细胞和用海藻酸钠包埋制作成的微囊化嗅鞘细胞移植至坐骨神经慢性损伤压迫模型大鼠损伤神经周围。于移植后第7，14 d进行机械缩足反射阈值检测和免疫组织化学染色检测大鼠L4-5背根神经节P2X2/3受体免疫反应。移植后第7，14天，嗅鞘细胞移植和微囊化嗅鞘细胞移植干预的坐骨神经损伤大鼠机械缩足反射阈值均增高，大鼠L4-5背根神经节P2X2/3受体的免疫阳性反应均减弱；且移植后第14 d，微囊化嗅鞘细胞移植干预的坐骨神经损伤大鼠变化更为明显。结果证实，微囊化嗅鞘细胞移植可抑制神经病理痛模型大鼠L4-5背根神经节P2X2/3受体的表达，从而减轻神经病理性疼痛的症状，效果优于嗅鞘细胞移植。

关键词: 神经再生, 周围神经损伤, 坐骨神经, 微囊, 嗅鞘细胞, P2X2/3 receptor, 神经病理痛, 背根神经节, 坐骨神经慢性损伤模型, 细胞移植, 国家自然科学基金

Abstract:

Olfactory bulb tissue transplantation inhibits P2X2/3 receptor-mediated neuropathic pain. However, the olfactory bulb has a complex cellular composition, and the mechanism underlying the action of purified transplanted olfactory ensheathing cells (OECs) remains unclear. In the present study, we microencapsulated OECs in alginic acid, and transplanted free and microencapsulated OECs into the region surrounding the injured sciatic nerve in rat models of chronic constriction injury. We assessed mechanical nociception in the rat models 7 and 14 days after surgery by measuring paw withdrawal threshold, and examined P2X2/3 receptor expression in L4–5 dorsal root ganglia using immunohistochemistry. Rats that received free and microencapsulated OEC transplants showed greater withdrawal thresholds than untreated model rats, and weaker P2X2/3 receptor immunoreactivity in dorsal root ganglia. At 14 days, paw withdrawal threshold was much higher in the microencapsulated OEC-treated animals. Our results confirm that microencapsulated OEC transplantation suppresses P2X2/3 receptor expression in L4–5 dorsal root ganglia in rat models of neuropathic pain and reduces allodynia, and also suggest that transplantation of microencapsulated OECs is more effective than transplantation of free OECs for the treatment of neuropathic pain.

Key words: nerve regeneration, peripheral nerve injury, sciatic nerve, microencapsulation, olfactory ensheathing cells, P2X2/3 receptor, neuropathic pain, dorsal root ganglion, sciatic chronic constriction injury, cell transplantation, NSFC grant, neural regeneration