中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (9): 1523-1527.doi: 10.4103/1673-5374.165527

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

弹性模量影响神经干细胞的生长及分化方向

  

  • 收稿日期:2015-06-26 出版日期:2015-09-28 发布日期:2015-09-28
  • 基金资助:

    国家自然科学基金青年项目(11102235);天津市科技支撑计划重点项目(14ZCZDGX00500);天津市自然科学基金重点项目(12JCZDJC24100);天津市卫生局科技基金项目(2013KZ134,2014KZ135);武警后勤学院附属医院种子基金项目(FYM201432)

Elastic modulus affects the growth and differentiation of neural stem cells

Xian-feng Jiang1, Kai Yang1, 2, Xiao-qing Yang1, Ying-fu Liu3, Yuan-chi Cheng1, 2, Xu-yi Chen1, Yue Tu1   

  1. 1 Affiliated Hospital of Logistics University of People’s Armed Police Force, Tianjin, China
    2 Liaoning Medical University, Jinzhou, Liaoning Province, China
    3 Logistics University of People’s Armed Police Force, Tianjin, China
  • Received:2015-06-26 Online:2015-09-28 Published:2015-09-28
  • Contact: Yue Tu, M.D. or Xu-yi Chen, M.D., ytumail@vip.126.com or chenxuyi1979@126.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation (Youth Project) of China, No. 11102235; a grant from the Key Project of Tianjin Science and Technology Support Plan in China, No. 14ZCZDGX00500; the Key Project of Natural Science Foundation of Tianjin City of China, No. 12JCZDJC24100; the Science and Technology Foundation Project of Tianjin Municipal Health Bureau of China, No. 2013KZ134, 2014KZ135; the Seed Foundation Project of Affiliated Hospital of Logistics University of People’s Armed Police Force of China, No. FYM201432.

摘要:

培养载体的硬度、弹性模量及接触面积等细胞生长的物理环境都是否会对神经干细胞的生长分化具有一定的影响。实验首先经拉伸试验得出天塑硅胶膜和SMI硅胶膜的弹性模量低于普通培养皿,且SMI硅胶膜低于天塑硅胶膜。然后提取孕16d SD大鼠胚胎大脑皮质神经干细胞。将神经干细胞分别接种于普通培养皿、天塑硅胶膜和SMI硅胶膜上培养。光镜可见3种细胞载体上的神经干细胞均能较好的贴壁生长。免疫荧光鉴定诱导分化7d后3种细胞中均可见神经特异性烯醇化酶、神经胶质纤维酸性蛋白和髓磷脂碱性蛋白阳性的细胞。流式细胞仪分析显示天塑硅胶膜和SMI硅胶膜上的神经干细胞向星形胶质细胞分化率高于普通培养皿,且SMI硅胶膜高于天塑硅胶膜。从而证实3种细胞载体均具有良好的生物相容性,SMI硅胶膜及天塑硅胶膜具有良好的力学均一性,弹性模量影响神经干细胞向不同神经细胞分化,在一定范围内,弹性模量越小,神经干细胞向星形胶质细胞分化趋势越明显

关键词: 神经再生, 神经干细胞, 载体, 力学性质, 弹性模量, 细胞分化, 神经元, 免疫荧光, 星形胶质细胞

Abstract:

It remains poorly understood if carrier hardness, elastic modulus, and contact area affect neural stem cell growth and differentiation. Tensile tests show that the elastic moduli of Tiansu and SMI silicone membranes are lower than that of an ordinary dish, while the elastic modulus of SMI silicone membrane is lower than that of Tiansu silicone membrane. Neural stem cells from the cerebral cortex of embryonic day 16 Sprague-Dawley rats were seeded onto ordinary dishes as well as Tiansu silicone membrane and SMI silicone membrane. Light microscopy showed that neural stem cells on all three carriers show improved adherence. After 7 days of differentiation, neuron specific enolase, glial fibrillary acidic protein, and myelin basic protein expression was detected by immunofluorescence. Moreover, flow cytometry revealed a higher rate of neural stem cell differentiation into astrocytes on Tiansu and SMI silicone membranes than on the ordinary dish, which was also higher on the SMI than the Tiansu silicone membrane. These findings confirm that all three cell carrier types have good biocompatibility, while SMI and Tiansu silicone membranes exhibit good mechanical homogenization. Thus, elastic modulus affects neural stem cell differentiation into various nerve cells. Within a certain range, a smaller elastic modulus results in a more obvious trend of cell differentiation into astrocytes. 

Key words: nerve regeneration, neural stem cells, carrier, mechanical properties, elastic modulus, cell differentiation, neurons, immunofluorescence, astrocytes, neural regeneration