中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (1): 107-113.doi: 10.4103/1673-5374.175054

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

高压氧联合脐带间充质干细胞移植修复脑创伤效果优于单一治疗方法

  

  • 收稿日期:2015-12-22 出版日期:2016-01-15 发布日期:2016-01-15

Umbilical cord-derived mesenchymal stem cell transplantation combined with hyperbaric oxygen treatment for repair of traumatic brain injury

Hai-xiao Zhou 1, Zhi-gang Liu 2, Xiao-jiao Liu 3, Qian-xue Chen 3   

  1. 1 Department of Plastic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
    2 Department of Anesthesiology, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
    3 Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
  • Received:2015-12-22 Online:2016-01-15 Published:2016-01-15
  • Contact: Hai-xiao Zhou, M.D.,zhxiaoo@163.com.

摘要:

临床上已有采用脐带间充质干细胞移植修复脑创伤的先例。高压氧疗法也早已被广泛用于辅助治疗颅脑创伤。因此,我们拟采用脐带间充质干细胞移植同时予以高压氧治疗创伤性脑损伤大鼠,以期达到更好的神经修复效果。实验建立重型液压颅脑创伤大鼠模型(2.5-3.0 atm液压冲击力)后,予以脐带间充质干细胞尾静脉注射移植联合高压氧治疗。发现与单独脐带间充质干细胞移植或高压氧治疗相比,大鼠损伤区脑组织水通道蛋白4表达降低,神经生长蛋白43表达升高,神经轴索样结构增多,CM-Dil阳性细胞数增高,认知和神经功能明显改善。结果提示,高压氧联合脐带间充质干细胞移植修复颅脑创伤效果优于两者单应用,能够明显促进神经功能的恢复。

关键词: 神经再生, 脑创伤, 脐带间充质干细胞, 移植, 高压氧, 大鼠, 颅脑创伤, 神经功能

Abstract:

Transplantation of umbilical cord-derived mesenchymal stem cells (UC-MSCs) for repair of traumatic brain injury has been used in the clinic. Hyperbaric oxygen (HBO) treatment has long been widely used as an adjunctive therapy for treating traumatic brain injury. UC-MSC transplantation combined with HBO treatment is expected to yield better therapeutic effects on traumatic brain injury. In this study, we established rat models of severe traumatic brain injury by pressurized fluid (2.5–3.0 atm impact force). The injured rats were then administered UC-MSC transplantation via the tail vein in combination with HBO treatment. Compared with monotherapy, aquaporin 4 expression decreased in the injured rat brain, but growth-associated protein-43 expression, calaxon-like structures, and CM-Dil-positive cell number increased. Following combination therapy, however, rat cognitive and neurological function significantly improved. UC-MSC transplantation combined with HBO therapyfor repair of traumatic brain injury shows better therapeutic effects than monotherapy and significantly promotes recovery of neurological functions.

Key words: nerve regeneration, traumatic brain injury, umbilical cord mesenchymal stem cells, transplantation, hyperbaric oxygen, rats, craniocerebral trauma, neurological function, neural regeneration