中国神经再生研究(英文版)

中国神经再生研究(英文版)

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

神经生长因子能有效促进树鼩海马神经干细胞的体外增殖

  

  • 收稿日期:2015-12-22 出版日期:2016-04-30 发布日期:2016-04-30
  • 基金资助:

    科技部国家重点科技研究与发展规划项目（2014BAI01B00）

Nerve growth factor promotes in vitro proliferation of neural stem cells from tree shrews

Liu-lin Xiong1, Zhi-wei Chen2, 3, Ting-hua Wang1, 2, 3, *   

  1. 1 Department of Anesthesiology, Institute of Neurological Disease, Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
    2 Institute of Neuroscience, Kunming Medical University, Kunming, Yunnan Province, China
    3 Key Laboratory of National Physical Fitness and Altitude Training Adaptation in Yunnan Province; Institute of Physical Education, Yunnan Normal University, Kunming, Yunnan Province, China
  • Received:2015-12-22 Online:2016-04-30 Published:2016-04-30
  • Contact: Ting-hua Wang, M.D., tinghua_neuron@263.net.
  • Supported by:

    This research was supported by a grant from the National Key Technology Research and Development Program of the Ministry of Science and Technology of China, No. 2014BAI01B00.

PDF

820

摘要：

干细胞虽然可促进神经再生以及神经组织修复，但是其来源和增殖能力有限，限制了其治疗效果。我们假设神经生长因子可促进与灵长类动物生理特征较为相似的树鼩神经干细胞的体外增殖。实验培养了来源于胚胎期38 d树鼩的海马组织神经干细胞，在培养基中增加100μg/L的神经生长因子，设置正常培养的树鼩的海马组织神经干细胞作为对照组，培养3 d后荧光显微镜检测发现，与对照组相比神经生长因子诱导组DAPI/Nestin荧光双标细胞数量明显增多，融合的细胞球数量也增多，细胞形态更完整。结果可以证实神经生长因子对树鼩神经干细胞增殖有明显促进作用。

orcid: 0000-0003-0775-9602 (Ting-hua Wang)

关键词: 神经再生, 树鼩, 海马, 神经干细胞, 细胞增殖, 神经生长因子, 神经球, 胚胎, 细胞数目, 细胞治疗, 体外实验

Abstract:

Neural stem cells promote neuronal regeneration and repair of brain tissue after injury, but have limited resources and proliferative ability
in vivo. We hypothesized that nerve growth factor would promote in vitro proliferation of neural stem cells derived from the tree shrews, a
primate-like mammal that has been proposed as an alternative to primates in biomedical translational research. We cultured neural stem
cells from the hippocampus of tree shrews at embryonic day 38, and added nerve growth factor (100 μg/L) to the culture medium. Neural
stem cells from the hippocampus of tree shrews cultured without nerve growth factor were used as controls. After 3 days, fluorescence microscopy
after DAPI and nestin staining revealed that the number of neurospheres and DAPI/nestin-positive cells was markedly greater in
the nerve growth factor-treated cells than in control cells. These findings demonstrate that nerve growth factor promotes the proliferation
of neural stem cells derived from tree shrews.

Key words: nerve regeneration, tree shrews, hippocampus, neural stem cells, cell proliferation, nerve growth factor, neurosphere, embryo, cell number, cell therapy, in vitro, neural regeneration