中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (4): 610-616.doi: 10.4103/1673-5374.180746

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

艾灸可促进海马颗粒蛋白前体表达

  

  • 收稿日期:2015-12-25 出版日期:2016-04-30 发布日期:2016-04-30
  • 基金资助:

    国家自然科学基金 (81303034 and 81303031), 中国博士后科学基金 (KLF501004)，上海中西医结合高峰科学发展项目

Moxibustion upregulates hippocampal progranulin expression

Tao Yi1, 2, Li Qi3, Ji Li1, 2, Jing-jing Le1, 2, Lei Shao1, 2, Xin Du1, 2, Jing-cheng Dong1, 2, *   

  1. 1 Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China
    2 College of Integrated Traditional Chinese and Western Medicine, Fudan University, Shanghai , China
    3 E-institute of Shanghai Municipal Education Committee, Shanghai University of Traditional Chinese Medicine, Shanghai, China
  • Received:2015-12-25 Online:2016-04-30 Published:2016-04-30
  • Contact: Jing-cheng Dong, Ph.D., jcdong2004@126.com.
  • Supported by:

    This study was funded by the National Natural Science Foundation (No. 81303034 and 81303031), China Postdoctoral Science Foundation (No. KLF501004) and Development Project of Shanghai Peak Disciplines-Integrated Chinese and Western Medicine.

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摘要：

已有研究显示，下丘脑-垂体-肾上腺轴功能异常是慢性应激导致慢性疲劳综合征产生的重要环节，艾灸治疗有效。为验证此学说，实验采用慢性束缚加强迫游泳法建立慢性疲劳综合征大鼠模型，选取关元(CV4)、双侧足三里(ST36)给予艾灸干预。强迫游泳实验、矿场实验及鼠尾悬挂实验、实时荧光定量PCR、ELISA检测、Western blot检测结果显示，艾灸降低了模型大鼠的下丘脑促肾上腺皮质激素释放激素mRNA表达，降低了外周血促肾上腺皮质激素、皮质酮含量，增加了海马颗粒蛋白前体mRNA及其蛋白表达。说明艾灸能通过调节下丘脑-垂体-肾上腺轴的功能，促进海马颗粒蛋白前体表达，发挥缓解慢性疲劳的作用。

orcid: 0000-0002-3645-0324 (Jing-cheng Dong)

关键词: 神经再生, 中医药, 艾灸, 慢性疲劳综合征, 行为学, 下丘脑-垂体-肾上腺轴, 促肾上腺皮质激素释放激素, 促肾上腺皮质激素, 皮质酮, 海马, 颗粒蛋白前体, 大鼠

Abstract:

In China, moxibustion is reported to be useful and has few side effects for chronic fatigue syndrome, but its mechanisms are largely unknown.
More recently, the focus has been on the wealth of information supporting stress as a factor in chronic fatigue syndrome, and
largely concerns dysregulation in the stress-related hypothalamic-pituitary-adrenal axis. In the present study, we aimed to determine the
effect of moxibustion on behavioral symptoms in chronic fatigue syndrome rats and examine possible mechanisms. Rats were subjected to
a combination of chronic restraint stress and forced swimming to induce chronic fatigue syndrome. The acupoints Guanyuan (CV4) and
Zusanli (ST36, bilateral) were simultaneously administered moxibustion. Untreated chronic fatigue syndrome rats and normal rats were
used as controls. Results from the forced swimming test, open field test, tail suspension test, real-time PCR, enzyme-linked immunosorbent
assay, and western blot assay showed that moxibustion treatment decreased mRNA expression of corticotropin-releasing hormone in
the hypothalamus, and adrenocorticotropic hormone and corticosterone levels in plasma, and markedly increased progranulin mRNA and
protein expression in the hippocampus. These findings suggest that moxibustion may relieve the behavioral symptoms of chronic fatigue
syndrome, at least in part, by modulating the hypothalamic-pituitary-adrenal axis and upregulating hippocampal progranulin.

Key words: nerve regeneration, traditional Chinese medicine, moxibustion, chronic fatigue syndrome, hypothalamic-pituitary-adrenal axis, corticotrophin-releasing hormone, adrenocorticotropic hormone, behavioral symptoms, corticosterone, hippocampus, progranulin, neural regeneration