中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (5): 829-834.doi: 10.4103/1673-5374.182712

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

自制低廉红外线系统评估小鼠坐骨神经功能

  

  • 收稿日期:2016-02-02 出版日期:2016-05-20 发布日期:2016-05-20

A self-made, low-cost infrared system for evaluating the sciatic functional index in mice

Lukas Fricker1, Vincenzo Penna1, Florian Lampert1, G. Bjoern Stark1, Christian Witzel2, #, Georgios Koulaxouzidis1, *, #   

  1. 1 Department of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, Germany 2 Plastic and Reconstructive Surgery, Charité – Universitätsmedizin Berlin, Berlin, Germany
  • Received:2016-02-02 Online:2016-05-20 Published:2016-05-20
  • Contact: Georgios Koulaxouzidis, M.D., georgios.koulaxouzidis@uniklinik-freiburg.de.

摘要:

以墨水和纸张获得足迹,从而得到坐骨神经功能指数是一种广泛用于周围神经功能评估的方法,但其存在墨点污迹及不能展示动态信息的缺点。如今发展起来的数字系统使坐骨神经功能评估更简单,但其价格昂贵,需要40000英磅。为此,我人研制了一种低廉的红外线系统用于评估小鼠坐骨神经功能指数。制备小鼠单侧坐骨神经挤压伤模型前及伤后2,4,6d,然后每天进行评估,直至伤后23d,共进行21次评估。以平衡木行走试验为参照,红外线系统评估的坐骨神经功能指数减少了33%的标准差。因此该系统检测次数和动物数量均有所减少,且其构建花费只需321英磅。实验结果证实,自制红外线系统用于坐骨神经功能指数评估,可获得更为精确的结果。

orcid: 0000-0001-5679-7686(Georgios Koulaxouzidis)

关键词: 神经再生, 坐骨神经, 挤压, 红外线, 平衡木行走试验, 错误率, 周围神经损伤

Abstract:

"The sciatic functional index (SFI) is a popular parameter for peripheral nerve evaluation that relies on footprints obtained with ink and paper. Drawbacks include smearing artefacts and a lack of dynamic information during measurement. Modern applications use digitized systems that can deliver results with less analytical effort and fewer mice. However, the systems are expensive (€40,000). This study aimed to evaluate the applicability and precision of a self-made, low-cost infrared system for evaluating SFI in mice. Mice were subjected to unilateral sciatic nerve crush injury (crush group; n = 7) and sham operation (sham group; n = 4). They were evaluated on the day before surgery, the 2nd, 4th and 6th days after injury, and then every day up to the 23rd day after injury. We compared two SFI evaluation methods, i.e., conventional inkand- paper SFI (C-SFI) and our infrared system (I-SFI). Our apparatus visualized footprints with totally internally reflected infrared light (950 nm) and a camera that can only detect this wavelength. Additionally we performed an analysis with the ladder beam walking test (LBWT) as a reference test. I-SFI assessment reduced the standard deviation by about 33 percent, from 11.6 to 7.8, and cut the variance around the baseline to 21 percent. The system thus requires fewer measurement repetitions and fewer animals, and cuts the cost of keeping the animals. The apparatus cost €321 to build. Our results show that the process of obtaining the SFI can be made more precise via digitization with a self-made, low-cost infrared system."

Key words: nerve regeneration, sciatic nerve, crush, frustrated total internal reflection, infrared, ladder beam walking test, error rate, mouse, peripheral nerve injury, neural regeneration