中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (7): 1099-1101.doi: 10.4103/1673-5374.187042

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针修复急性脑缺血再灌注损伤的机制：可能与血清中转化生长因子β1 表达上调有关

  

  • 出版日期:2016-07-30 发布日期:2016-07-30
  • 基金资助:
    河南医药科技重点项目，河南省卫生厅项目（200902009）

Mechanisms of electroacupuncture effects on acute cerebral ischemia/reperfusion injury: possible association with upregulation of transforming growth factor beta 1

Wen-biao Wang1, *, Lai-fu Yang1, Qing-song He1, Tong Li2, Yi-yong Ma1, Ping Zhang2, Yi-sheng Cao1   

  1. 1 Department of Rehabilitation, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China 2 Department of Neurology, the First Affiliated Hospital of Xinxiang Medical College, Weihui, Henan Province, China
  • Online:2016-07-30 Published:2016-07-30
  • Contact: Wen-biao Wang, M.D., wenbiaowang7306@163.com.
  • Supported by:
    This study was supported by the Key Project Fund of Henan Medical Science and Technology of Henan Province Health Bureau in China, No. 200902009.

PDF

385

摘要：

临床研究已证实，电针百会(GV 20)和水沟(GV 26)头穴可促进缺血性脑血管疾病患者的神经功能恢复，但机制尚不清楚。实验假设电针刺激头部穴位修复急性脑缺血再灌注损伤与转化生长因子β1 的表达有关。为此，我们采用大脑中动脉栓塞法建立脑缺血模型大鼠，对其百会(GV 20)和水沟(GV 26)头穴(疏密波2或5 Hz, 3 mA，30 min/次)电针干预，干预12，24，48，72 h。以神经功能缺损评分和酶联免疫吸附试验检测结果显示，电针干预的模型大鼠神经功能缺损评分降低，神经功能缺损评分血清中转化生长因子β1的表达上调。结果说明电针百会(GV 20)和水沟(GV 26)头穴可上调急性脑缺血再灌注损伤大鼠血清中转化生长因子β1 表达，以此发挥神经保护作用。 

orcid: 0000-0002-2910-1361 (Wen-biao Wang) 

关键词: 神经再生, 中医药, 脑损伤, 电针, 脑缺血, 再灌注损伤, 转化生长因子&beta, 1, 神经损伤, 神经功能评分, 神经功能康复, 炎症反应

Abstract:

Electroacupuncture at the head acupoints Baihui (GV20) and Shuigou (GV26) improves recovery of neurological function following ischemic cerebrovascular events, but its mechanism remains incompletely understood. We hypothesized that the action of electroacupuncture at these acupoints is associated with elevated serum levels of transforming growth factor beta 1 (TGF-β1). To test this, we established a rat model of cerebral ischemia by middle cerebral artery occlusion. Electroacupuncture was performed at Baihui and Shuigou with a “dispersedense” wave at an alternating frequency of 2 and 150 Hz, and at a constant intensity of 3 mA. Each electroacupuncture session lasted 30 minutes and was performed every 12 hours for 3 days. Neurological severity scores were lower in injured rats after acupuncture than in those not subjected to treatment. Furthermore, serum level of TGF-β1 was greater after electroacupuncture than after no treatment. Our results indicate that electroacupuncture at Baihui and Shuigou increases the serum level of TGF-β1 in rats with acute cerebral ischemia/ reperfusion injury, and exerts neuroprotective effects.

Key words: nerve regeneration, traditional Chinese medicine, brain injury, cerebral ischemia, reperfusion, transforming growth factor-beta 1, nerve injury, neurological severity score, neurofunctional rehabilitation, inflammatory reaction, neural regeneration