中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (8): 1304-1311.doi: 10.4103/1673-5374.189196

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

姜黄素促进完全离断坐骨神经的修复:与脊髓组织S100表达上调有关?

  

  • 出版日期:2016-08-31 发布日期:2016-08-31
  • 基金资助:
    吉林省科技发展项目(20150311038YY)

Curcumin upregulates S100 expression and improves regeneration of the sciatic nerve following its complete amputation in mice

Guo-min Liu1, Kun Xu2, Juan Li2, Yun-gang Luo3, *   

  1. 1 Department of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China 2 Department of Health Laboratory, School of Public Health, Jilin University, Changchun, Jilin Province, China 3 Department of Stomatology, the Second Hospital of Jilin University, Changchun, Jilin Province, China
  • Online:2016-08-31 Published:2016-08-31
  • Contact: Yun-gang Luo, M.D., luoygjlu@sina.com.
  • Supported by:
    This research was supported by the Jilin Provincial Science & Technology Development Project Fund of China, No. 20150311038YY

摘要:

完全离断损伤的周围神经修复较为困难,仅靠吻合手术难以达到快速恢复神经功能的效果。研究表明,姜黄素能够有效修复大鼠坐骨神经的挤压性损伤。为验证此作用及机制,我们设计的实验将BALB/c小鼠坐骨神经完全离断后立即进行神经外膜吻合,姜黄素灌胃1周后,在麻醉状态下取损伤小鼠坐骨神经进行固蓝染色和电生理检测,取L4-6脊髓节段组织进行免疫组织化学染色、实时PCR和Western blot检测发现,以40,20 mg/(kg.d)姜黄素灌胃的小鼠坐骨神经髓鞘形状规则、厚度均匀、边界清晰、周围髓增生少,坐骨神经动作电位幅度和运动神经传导速率明显改善,L4-6脊髓节段许旺细胞增殖的标志物S100的免疫反应、mRNA和蛋白表达均上调。结果表明,姜黄素干预可有效促进坐骨神经完全离断损伤的修复,其具体机制可能与S100表达上调有关。 

orcid: 0000-0002-5075-2759 (Yun-gang Luo)

关键词: 神经再生, 周围神经损伤, 姜黄素, S100, 坐骨神经, 周围神经再生, 动作电位, 神经功能

Abstract: The repair of peripheral nerve injury after complete amputation is diffcult, and even with anastomosis, the rapid recovery of nerve function remains challenging. Curcumin, extracted from plants of the genus Curcuma, has been shown to have anti-oxidant and anti-in?ammatory properties and to improve sciatic nerve crush injury in rats. Here, we determined whether curcumin had neuroprotective effects following complete peripheral nerve amputation injury. BALB/c mice underwent complete sciatic nerve amputation, followed by an immediate epineurium anastomosis. Mice were intragastrically administered curcumin at doses of 40 (high), 20 (moderate), and 10 mg/kg/d (low) for 1 week. We found that myelin in the mice of the high- and moderate-dose curcumin groups appeared with regular shape, uniform thickness, clear boundary, and little hyperplasia surrounding the myelin. High and moderate doses of curcumin markedly improved both action potential amplitude of the sciatic nerves and the conduction velocity of the corresponding motor neurons, and upregulated mRNA and protein expression of S100, a marker for Schwann cell proliferation, in L4–6 spinal cord segments. These results suggest that curcumin is effective in promoting the repair of complete sciatic nerve amputation injury and that the underlying mechanism may be associated with upregulation of S100 expression.

Key words: nerve regeneration, electrophysiology, complete nerve amputation, spinal cord, myelin sheath, myelinated fiber, epineurium anatomosis, neural regeneration