中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (8): 1339-1346.doi: 10.4103/1673-5374.189201

• 原著:退行性病与再生 • 上一篇    下一篇

B3C和雷沙吉兰联合使用没有协同保护帕金森病小鼠多巴胺能神经元

  

  • 出版日期:2016-08-31 发布日期:2016-08-31
  • 基金资助:
    广东省自然科学基金项目（2015A030313317），广州市科技项目（2014J4100097），中国澳门特别行政区科技发展基金（134/2014/A3），中国澳门大学研究委员会项目（MYRG139(Y1-L4)-ICMS12-LMY，MYRG2015-00214-ICMS-QRCM），中国香港特别行政区研究资助局项目（561011, 15101014），中国香港理工大学项目（G-SB10，G-UC15，G-YBGQ）

No synergism between bis(propyl)-cognitin and rasagiline on protecting dopaminergic neurons in Parkinson’s disease mice

Cheng-you Zheng1, Bao-jian Guo1, Wei Cai1, Wei Cui2, Shing-hung Mak3, Yu-qiang Wang1, Simon Ming-yuen Lee4, Yi-fan Han3, *, Zai-jun Zhang1, *   

  1. 1 Institute of New Drug Research and Guangzhou Key Laboratory of Innovative Chemical Drug Research in Cardiocerebrovascular Diseases, Jinan University College of Pharmacy, Guangzhou, Guangdong Province, China 2 School of Medicine, Ningbo University, Ningbo, Zhejiang Province, China 3 Department of Applied Biology and Chemical Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, Hung Hom, Hong Kong Special Administrative Region, China 4 State Key Laboratory of Quality Research of Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Taipa, Macao Special Administrative Region, China
  • Online:2016-08-31 Published:2016-08-31
  • Contact: Zai-jun Zhang, M.D. or Yi-fan Han, zaijunzhang@163.com orbcyfhan@polyu.edu.hk.
  • Supported by:
    This study was supported by grants from the Natural Science Foundation of Guangdong Province of China, No. 2015A030313317; a grant from the Science and Technology Program of Guangzhou City of China, No. 2014J4100097; and partially by a grant from the Science and Technology Development Fund (FDCT) of Macao Special Administrative Region, No. 134/2014/A3; a grant from the Research Committee of University of Macau, No. MYRG139(Y1-L4)-ICMS12-LMY and MYRG2015-00214-ICMS-QRCM; and grants from the Research Grants Council of Hong Kong Special Administrative Region of China, No. 561011, 15101014; the Hong Kong Polytechnic University of China, No. G-SB10, G-UC15 and G-YBGQ

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摘要：

单胺氧化酶B抑制剂雷沙吉兰和新型乙酰胆碱酯酶抑制剂B3C都具有良好的神经保护和神经修复作用，两者对帕金森病是否存在协同作用呢？实验对MPTP诱导的帕金森病模型小鼠灌胃B3C和/或雷沙吉兰，观察模型小鼠神经行为学、高效液相色谱法和Western blot的结果发现，B3C和/或雷沙吉兰都能改善MPTP诱导小鼠的行为障碍，增加其大脑纹状体多巴胺及其代谢产物的水平，上调黑质中酪氨酸羟化酶的表达，但B3C和雷沙吉兰两者联合灌胃的效果并不比单独使用一种的效果更好。因此认为以0.3 mg/kg B3C和0.02 mg/kg雷沙吉兰联合使用没有协同的神经保护作用。 

orcid: 0000-0002-0690-1673 (Zai-jun Zhang)

关键词: 神经再生, 帕金森病, B3C, 雷沙吉兰, 单胺氧化酶, 单胺氧化酶B, 多靶点, 协同作用, 神经保护

Abstract: Rasagiline, a monoamine oxidase-B inhibitor, and bis(propyl)-cognitin (B3C), a novel dimer are reported to be neuroprotective. Herein, the synergistical neuroprotection produced by rasagiline and B3C was investigated in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice of Parkinsonism. By using neurobehavioural tests, high-performance liquid chromatography and western blot assay, we showed that B3C at 0.3 mg/kg, rasagiline at 0.02 mg/kg, as well as co-treatment with B3C and rasagiline prevented MPTP-induced behavioural abnormities, increased the concentrations of dopamine and its metabolites in the striatum, and up-regulated the expression of tyrosine hydroxylase in the substantia nigra. However, the neuroprotective effects of co-treatment were not signifcantly improved when compared with those of B3C or rasagiline alone. Collectively, we have demonstrated that B3C at 0.3 mg/kg and rasagline at 0.02 mg/kg could not produce synergistic neuroprotective effects.

Key words: nerve regeneration, Parkinson’s disease, bis(propyl)-cognitin, rasagiline, monoamine oxidase B, dopamine, multitarget, synergism; neuroprotection, neural regeneration