中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (9): 1471-1479.doi: 10.4103/1673-5374.191222

• 原著:退行性病与再生 • 上一篇    下一篇

急性精神应激条件下5-羟色胺可调控脑组织中脑源性神经营养因子的表达

  

  • 收稿日期:2016-08-19 出版日期:2016-09-30 发布日期:2016-09-30
  • 基金资助:
    山东省自然科学基金(ZR2011HM023),十一五计划项目(2007BAI17B02),山东省高校科技项目(J10LF01),山东省医学科技发展项目(2011HZ011), 中国博士后科研基金(2012M 520585),天津市卫生厅基金(2014KR02), 海南Li Ou制药基金，江苏恩华药业集团有限公司基金项目

Serotonin regulates brain-derived neurotrophic factor expression in select brain regions during acute psychological stress

De-guo Jiang1, #, Shi-li Jin2, #, Gong-ying Li2, 3, #, Qing-qing Li3, Zhi-ruo Li2, Hong-xia Ma3, Chuan-jun Zhuo1, 3, 4, *, Rong-huan Jiang5, *, Min-jie Ye6, *   

  1. 1 Department of Psychiatry, Wenzhou 7th People’s Hospital, Wenzhou, Zhejiang Province, China 2 Department of Psychiatry, Second Affliated Hospital of Jining Medical University, Jining, Shandong Province, China 3 Department of Psychiatry, Jining Medical University, Jining, Shandong Province, China 4 Department of Psychiatry, Tianjin Anding Hospital, Tianjin, China 5 Department of Psychological Medicine, Chinese PLA General Hospital, Department of Psychological Medicine, Chinese PLA Medical School, Beijing, China 6 The Affliated Kangning Hospital of Wenzhou Medical University, Wenzhou, Zhejiang Province, China
  • Received:2016-08-19 Online:2016-09-30 Published:2016-09-30
  • Contact: Chuan-jun Zhuo, Rong-huan Jiang, M.D. or Min-jie Ye, Ph.D., jiangrh55@126.com or mjyewmkhospital@163.com.
  • Supported by:
    This work was supported by grants from the Natural Science Foundation of Shandong Province of China (ZR2011HM023 to GYL), the “11th Five-Year Plan”, National Supporting Program (2007BAI17B02 to GYL), the Science and Technology Project of Higher Education of Shandong Province of China (J10LF01 to GYL), a grant from Medical Science and Technology Development Project of Shandong Province of China (2011HZ011 to GYL), the China Postdoctoral Science Foundation of China (2012M 520585 to CJZ), the Fund of Tianjin Health Bureau of China (2014KR02 to CJZ), the Foundation of Hainan Li Ou Pharmaceutical Co., Ltd. and the Foundation of Xuzhou Enhua Pharmaceutical Co., Ltd. of China.

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摘要：

既往研究显示，5-羟色胺可与脑源性神经营养因子在拮抗应激中存在相互作用，但两者在单纯精神应激条件下的关系尚未见报道。实验在大鼠急性精神应激前1h，给予腹腔注射5-羟色胺1A受体激动剂8-羟基-2-(双-正丙胺基)-四氢萘满或拮抗剂MDL73005干预，腹腔注射5-羟色胺2A受体激动剂DOI或拮抗剂酮舍林干预。原位杂交和免疫组化结果显示，5-羟色胺1A受体和5-羟色胺2A受体受体激动剂干预的大鼠，在大脑海马CA1区、CA3区及齿状回、前额皮质、中央杏仁核、丘脑背内侧核、伏隔核壳区和中脑导水管周围灰质中脑源性神经营养因子mRNA和免疫反应相近，且明显高于单纯精神应激大鼠；5-羟色胺1A受体和5-羟色胺2A受体拮抗剂干预的大鼠，大脑海马CA1区、CA3区及齿状回、前额皮质、中央杏仁核、丘脑背内侧核、伏隔核壳区和中脑导水管周围灰质中脑源性神经营养因子mRNA和免疫反应低于单纯精神应激大鼠。说明5-羟色胺参与急性精神应激后脑源性神经营养因子表达的调控。 

orcid: 0000-0002-9229-1591 (Min-jie Ye) 0000-0002-5023-1259 (Rong-huan Jiang)

关键词: 神经再生, 精神应激, 5-羟色胺, 5-羟色胺1A受体, 5-羟色胺2A受体, 脑源性神经营养因子

Abstract: Previous studies suggest that serotonin (5-HT) might interact with brain-derived neurotrophic factor (BDNF) during the stress response. However, the relationship between 5-HT and BDNF expression under purely psychological stress is unclear. In this study, one hour before psychological stress exposure, the 5-HT1A receptor agonist 8-OH-DPAT or antagonist MDL73005, or the 5-HT2A receptor agonist DOI or antagonist ketanserin were administered to rats exposed to psychological stress. Immunohistochemistry and in situ hybridization revealed that after psychological stress, with the exception of the ventral tegmental area, BDNF protein and mRNA expression levels were higher in the 5-HT1A and the 5-HT2A receptor agonist groups compared with the solvent control no-stress or psychological stress group in the CA1 and CA3 of the hippocampus, prefrontal cortex, central amygdaloid nucleus, dorsomedial hypothalamic nucleus, dentate gyrus, shell of the nucleus accumbens and the midbrain periaqueductal gray. There was no signifcant difference between the two agonist groups. In contrast, after stress exposure, BDNF protein and mRNA expression levels were lower in the 5-HT1A and 5-HT2A receptor antagonist groups than in the solvent control non-stress group, with the exception of the ventral tegmental area. Our fndings suggest that 5-HT regulates BDNF expression in a rat model of acute psychological stress.

Key words: nerve regeneration, psychological stress, serotonin, 5-HT1A, 5-HT2A, brain-derived neurotrophic factor, neural regeneration