中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (9): 1517-1526.doi: 10.4103/1673-5374.191228

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

促生长激素神经肽及其受体系统促进糖尿病大鼠损伤坐骨神经的修复

  

  • 收稿日期:2016-08-15 出版日期:2016-09-30 发布日期:2016-09-30
  • 基金资助:
    国家自然科学基金资助项目(31440047)和广东省省自然科学基金项目(2015a030310152)

Galanin and its receptor system promote the repair of injured sciatic nerves in diabetic rats

Xiao-feng Xu1, *, Dan-dan Zhang2, Jin-chi Liao1, Li Xiao1, Qing Wang1, Wei Qiu1   

  1. 1 The Third Affliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong Province, China 2 South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, Guangdong Province, China
  • Received:2016-08-15 Online:2016-09-30 Published:2016-09-30
  • Contact: Xiao-feng Xu, M.D., Ph.D., xuxf5@mail.sysu.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 31440047; the Natural Science Foundation of Guangdong Province in China, No. 2015A030310152.

摘要:

已经有研究证实促生长激素神经肽可在体外促进背根神经节神经元的轴突再生,并抑制神经病理性疼痛,但有关其对糖尿病周围神经病变的影响作用研究较少,尚缺乏体内实验来证实。我们假设体内长期给予外源性促生长激素神经肽也可促进糖尿病周围神经病变损伤的周围神经修复,并减轻疼痛。结果发现,链唑毒霉素素诱导的糖尿病大鼠坐骨神经损伤后,可出现严重的神经病理性疼痛。外源性促生长激素神经肽连续干预14,28 d,可使其神经病理性疼痛减轻并促进损伤坐骨神经修复,伴随背根神经节和脊髓后角的内源性促生长激素神经肽及其受体1、2在背根神经节和脊髓后角的表达改变。结果表明,长期连续应用促生长激素神经肽28 d,可促进糖尿病大鼠损伤坐骨神经的修复,并减轻神经病理性疼痛。 

orcid: 0000-0002-8413-3278 (Xiao-feng Xu)

关键词: 神经再生, 周围神经损伤, 糖尿病, 坐骨神经, 促生长激素神经肽, 促生长激素神经肽受体1, 促生长激素神经肽受体2, 神经病理性疼痛, 背根神经节, 脊髓后角

Abstract: Various studies have reported that galanin can promote axonal regeneration of dorsal root ganglion neurons in vitro and inhibit neuropathic pain. However, little is known about its effects on diabetic peripheral neuropathy, and in vivo experimental data are lacking. We hypothesized that repeated applications of exogenous galanin over an extended time frame may also repair nerve damage in diabetic peripheral neuropathy, and relieve pain in vivo. We found that neuropathic pain occurred in streptozotocin-induced diabetic rats and was more severe after sciatic nerve pinch injury at 14 and 28 days than in diabetic sham-operated rats. Treatment with exogenous galanin alleviated the neuropathic pain and promoted sciatic nerve regeneration more effectively in diabetic rats than in non-diabetic rats after sciatic nerve pinch injury. This was accompanied by changes in the levels of endogenous galanin, and its receptors galanin receptor 1 and galanin receptor 2 in the dorsal root ganglia and the spinal dorsal horn when compared with nerve pinch normal rats. Our results show that application of exogenous galanin daily for 28 days can promote the regeneration of injured sciatic nerves, and alleviate neuropathic pain in diabetic rats.

Key words: nerve regeneration, peripheral nerve injury, diabetes, sciatic nerve, galanin, galanin receptor 1, galanin receptor 2, neuropathic pain, dorsal root ganglion, spinal dorsal horn, neural regeneration