中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 1976-1980.doi: 10.4103/1673-5374.197141

• 原著:退行性病与再生 • 上一篇    下一篇

杨梅黄酮能保护阿尔茨海默病大鼠海马CA3区神经元和改善学习记忆功能

  

  • 收稿日期:2016-11-29 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    伊朗Arak大学科学部生物学系基金(38156-8-8349)

Myricetin protects hippocampal CA3 pyramidal neurons and improves learning and memory impairments in rats with Alzheimer’s disease

Matin Ramezani1, 2, *, Niloufar Darbandi1, Fariba Khodagholi2, Azam Hashemi1   

  1. 1 Department of Biology, Faculty of Science, Arak University, Arak, Iran 2 Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Received:2016-11-29 Online:2016-12-31 Published:2016-12-31
  • Contact: Matin Ramezani, M.S.c., matinramezani89@gmail.com.
  • Supported by:
    The study was funded by the Department of Biology, Faculty of Science, Arak University, Iran, No. 38156-8-8349.

摘要:

目前尚缺乏可有效延缓阿尔茨海默病病程的方法,因而该病的早期预防显得尤为重要。以往已有研究证实,黄酮类物质可以改善记忆功能。实验为验证杨梅黄酮是否有此作用,给予大鼠侧脑室内注射链脲佐霉素建立阿尔茨海默病模型后,连续21d按5和10 mg/kg剂量腹腔注射杨梅黄酮,给药结束后应用HE染色观察大鼠海马CA3区神经元形态变化,以穿梭箱实验定量测定大鼠学习记忆功能。结果显示,杨梅黄酮提高海马CA3区锥体细胞层神经元数量,明显改善阿尔茨海默病大鼠学习记忆功能。由此说明杨梅黄酮有治疗阿尔茨海默病的潜力。

orcid: 0000-0003-0218-968X (Matin Ramezani)

关键词: 神经再生, 杨梅黄酮, 阿尔茨海默病, 链脲佐霉素, 海马, CA3区, 行为学测验

Abstract: There is currently no treatment for effectively slowing the progression of Alzheimer’s disease, so early prevention is very important. Numerous studies have shown that ?avonoids can improve memory impairment. Te present study investigated the effects of myricetin, a member of the ?avonoids, on intracerebroventricular streptozotocin induced neuronal loss and memory impairment in rat models of Alzheimer’s disease. Myricetin at 5 or 10 mg/kg was intraperitoneally injected into rats over 21 days. Control rats were treated with 10 mL/kg saline. Behavioral test (the shuttle box test) was performed on day 22 to examine learning and memory in rats. Immediately after that, hematoxylin-eosin staining was performed to observe the morphological change in hippocampal CA3 pyramidal neurons. Myricetin greatly increased the number of hippocampal CA3 pyramidal neurons and improved learning and memory impairments in rats with Alzheimer’s disease. Tese fndings suggest that myricetin is benefcial for treatment of Alzheimer’s disease.

Key words: nerve regeneration, myricetin, Alzheimer’s disease, streptozotocin, hippocampus, pyramidal neurons, CA3 region, behavioral test, neural regeneration