中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (12): 1990-1996.doi: 10.4103/1673-5374.197143

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

精细技巧性功能训练可增强脊髓损伤后皮质脊髓束功能可塑性

  

  • 收稿日期:2016-11-07 出版日期:2016-12-31 发布日期:2016-12-31
  • 基金资助:
    国家自然科学基金(30972153)

Fine motor skill training enhances functional plasticity of the corticospinal tract afer spinal cord injury

Jian Liu, Xiao-yu Yang*, Wei-wei Xia, Jian Dong, Mao-guang Yang, Jian-hang Jiao   

  1. Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
  • Received:2016-11-07 Online:2016-12-31 Published:2016-12-31
  • Contact: Xiao-yu Yang, M.D., yangxiaoyu88@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, No. 30972153.

摘要:

损伤中枢神经后可通过神经可塑性在远离损伤部位出现轴突发芽并延伸长入失神经支配区,从而重建神经环路,如何提高这种神经可塑性成为中枢神经系统损伤修复成功的关键。目前研究表明,脊髓损伤后一些精细技巧性功能运动训练是否更有利于神经功能恢复仍存在争议。为此,实验采用锥体束切断法建立皮质脊髓束精确单侧损伤模型大鼠,于损伤前2周和损伤后第3天进行水平阶梯爬行和食物小球抓取的精细技巧性功能训练。损伤后1,2,3,4,6周,对大鼠进行前肢神经功能评估,并利用生物素葡聚糖胺顺行示踪健侧皮质脊髓束不同时间段长入失神经支配区轴突的生长情况。结果发现,与未训练模型大鼠比较,经训练的大鼠损伤后3周,大鼠患侧前肢和前爪功能恢复较好,损伤脊髓区皮质脊髓束轴突生长数量及生长相关蛋白43表达明显增多。说明精细技巧性功能训练对于脊髓损伤后大鼠中枢神经可塑性改变具有明显的促进作用。 

orcid: 0000-0001-9388-3794 (Xiao-yu Yang)

关键词: 神经再生, 脊髓损伤, 可塑性, 轴突, 功能锻炼, 皮质脊髓束, 生长相关蛋白43

Abstract: Following central nervous system injury, axonal sprouts form distal to the injury site and extend into the denervated area, reconstructing neural circuits through neural plasticity. How to facilitate this plasticity has become the key to the success of central nervous system repair. It remains controversial whether fne motor skill training contributes to the recovery of neurological function afer spinal cord injury. Terefore, we established a rat model of unilateral corticospinal tract injury using a pyramidal tract cutting method. Horizontal ladder crawling and food ball grasping training procedures were conducted 2 weeks before injury and 3 days afer injury. Te neurological function of rat forelimbs was assessed at 1, 2, 3, 4, and 6 weeks afer injury. Axon growth was observed with biotinylated dextran amine anterograde tracing in the healthy corticospinal tract of the denervated area at different time periods. Our results demonstrate that compared with untrained rats, functional recovery was better in the forelimbs and forepaws of trained rats. Te number of axons and the expression of growth associated protein 43 were increased at the injury site 3 weeks afer corticospinal tract injury. Tese fndings confrm that fne motor skill training promotes central nervous system plasticity in spinal cord injury rats.

Key words: nerve regeneration, spinal cord injury, plasticity, axons, functional training, corticospinal tract, growth associated protein 43, neural regeneration