中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2017, Vol. 12 ›› Issue (8): 1322-1328.doi: 10.4103/1673-5374.213553

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

冷水游泳预处理改善创伤性脑损伤大鼠的认知功能障碍

  

  • 收稿日期:2017-05-25 出版日期:2017-08-15 发布日期:2017-08-15
  • 基金资助:

    天津医科大学总医院在中国自然科学基金（303071901401）号自然科学基金孵化项目的资金支持；天津市自然科学基金（13jczdjc30800）；国家自然科学基金（81271361，81330029）。

Cold water swimming pretreatment reduces cognitive deficits in a rat model of traumatic brain injury

Zi-wei Zhou1, 2, 3, 4, Ya-dan Li5, Wei-wei Gao1, 2, 3, 4, Jie-li Chen6, Shu-yuan Yue1, 2, 3, 4, Jian-ning Zhang1, 2, 3, 4   

  1. 1 Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin, China;          
    2 Tianjin Neurological Institute, Tianjin, China;
    3 Key Laboratory of Post-trauma Neuro-repair and Regeneration in Central Nervous System, Ministry of Education, Tianjin, China;
    4 Tianjin Key Laboratory of Injuries, Variations and Regeneration of Nervous System, Tianjin, China;
    5 Intensive Care Units, Tianjin Huanhu Hospital, Tianjin, China;       
    6 Department of Neurology, Henry Ford Hospital, Detroit, MI, USA
  • Received:2017-05-25 Online:2017-08-15 Published:2017-08-15
  • Contact: Zi-wei Zhou or Jian-ning Zhang,1985zhouziwei@163.com or jianningzhang@hotmail.com.
  • Supported by:

    This study was supported by a grant from the Incubation Project of Natural Science Foundation of Tianjin Medical University General Hospital in China, No. 303071901401; the Natural Science Foundation of Tianjin of China, No. 13JCZDJC30800; the National Natural Science Foundation of China, No. 81271361 and 81330029.

PDF

367

摘要：

 

适度的应激刺激如冷水游泳，可提高机体对恶劣事件的耐受性，然而这种适度应激带来益处的机制尚不明确。为此，实验设计建立冲击损伤脑创伤大鼠模型，假设冷水游泳预处理可促进其脑创伤后认知功能的恢复，并探讨其机制。结果发现脑创伤后应用冷水游泳预处理(4℃冷水中游泳3 min)的大鼠与未经冷水预处理的脑损伤大鼠相比，其在Morris水迷宫的寻找目标象限时间百分率显著增高，逃避潜伏期更短。脑创伤后的0，3，6，24 h，冷水游泳预处理的大鼠较未预处理的大鼠外周循环血中有更多的内皮祖细胞。免疫组织化学染色及VWF染色发现，脑创伤后冷水游泳预处理大鼠的损伤侧海马组织的内皮祖细胞(CD34阳性细胞)及新生血管数量均增多。说明冷水游泳预处理可促进外周血及海马组织内皮祖细胞增殖及血管新生，以此产生改善创伤性脑损伤后认知缺陷的作用。

 

orcid:0000-0002-5927-7835(Zi-wei Zhou)
0000-0002-7290-0994(Jian-ning Zhang)

关键词: 神经再生, 冷水游泳, 认知缺陷, 内皮祖细胞, 血管, 神经修复, 应激, Morris水迷宫, 冲击损伤模型, CD34, vWF

Abstract:

A moderate stress such as cold water swimming can raise the tolerance of the body to potentially injurious events. However, little is  known about the mechanism of beneficial effects induced by moderate stress. In this study, we used a classic rat model of traumatic brain injury to test the hypothesis that cold water swimming preconditioning improved the recovery of cognitive functions and explored the mechanisms. Results showed that after traumatic brain injury, pre-conditioned rats (cold water swimming for 3 minutes at 4°C) spent a significantly higher percent of times in the goal quadrant of cold water swim, and escape latencies were shorter than for non-pretreated rats. The number of circulating endothelial progenitor cells was significantly higher in pre-conditioned rats than those without pretreatment at 0, 3, 6 and 24 hours after traumatic brain injury. Immunohistochemical staining and Von Willebrand factor staining demonstrated that the number of CD34+ stem cells and new blood vessels in the injured hippocampus tissue increased significantly in pre-conditioned rats. These data suggest that pretreatment with cold water swimming could promote the proliferation of endothelial progenitor cells and angiogenesis in the peripheral blood and hippocampus. It also ameliorated cognitive deficits caused by experimental traumatic brain injury.

Key words: nerve regeneration, cold water swimming, cognitive deficits, endothelial progenitor cells, angiogenesis, neural repair, stress, Morris water maze, fluid percussion injury model, CD34, Von Willebrand factor, neural regeneration