中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (1): 128-134.doi: 10.4103/1673-5374.217349

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

芦丁保护损伤脊髓神经的机制：通过抗氧化，抗炎和抑制p38 MAPK通路实现？

  

  • 收稿日期:2017-07-15 出版日期:2018-01-15 发布日期:2018-01-15
  • 基金资助:

    中国山东省青年科学家奖基金会（BS2013YY049）；中国博士后科学基金（2012M511036）

Neuroprotective mechanisms of rutin for spinal cord injury through anti-oxidation and anti-inflammation and inhibition of p38 mitogen activated protein kinase pathway.

Hong-liang Song1, Xiang Zhang2, Wen-zhao Wang1, Rong-han Liu1, Kai Zhao1, Ming-yuan Liu3, Wei-ming Gong1, Bin Ning1   

  1. 1 Department of Spinal Surgery, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China
    2 Hospital Pharmacy, Jinan Central Hospital Affiliated to Shandong University, Jinan, Shandong Province, China
    3 Department of Gynecology and Obstetrics, Jinan Maternity and Child Care Hospital, Jinan, Shandong Province, China
  • Received:2017-07-15 Online:2018-01-15 Published:2018-01-15
  • Contact: Bin Ning, ningbin@sdu.edu.cn.
  • Supported by:

    This work was supported in part by grants from the Young Scientists Awards Foundation of Shandong Province of China, No. BS2013YY049; and the China Postdoctoral Science Foundation, No. 2012M511036.

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摘要：

黄酮类物质芦丁具有抗炎、抗氧化、抗病毒、抗肿瘤和免疫调节作用。但是，芦丁对脊髓损伤的神经保护作用目前尚无深入研究。p38丝裂原活化蛋白激酶(p38 MAPK)通路是MAPK家族控制炎症反应最重要的成员，我们假设芦丁参与修复脊髓损伤的机制与抑制p38 MAPK通路有关。采用Allen打击法建立脊髓挫伤大鼠模型，给予芦丁(30 mg/kg)腹腔注射3 d，发现大鼠BBB评分增加，脊髓组织含水量、炎性因子肿瘤坏死因子α、白细胞介素1β及6水平、脊髓T8-9段p38MAPK蛋白表达和半胱天冬酶3，9活性均降低，而外周血氧化应激相关标记物超氧化物歧酶及谷胱甘肽过氧物酶水平升高。说明芦丁对脊髓损伤的神经保护机制是通过抗氧化，抗炎和抑制p38 MAPK通路实现的。

orcid:0000-0002-7592-9485(Bin Ning)

关键词: 神经再生, 脊髓损伤, 芦丁, 氧化应激, 抗氧化作用, 抗炎作用, p38 MAPK通路, 抗细胞凋亡作用, Caspase 3, Caspase 9

Abstract:

Rutin has anti-inflammatory, antioxidant, anti-viral, anti-tumor and immune regulatory effects. However, the neuroprotective effects of rutin in spinal cord injury are unknown. The p38 mitogen activated protein kinase (p38 MAPK) pathway is the most important member of the MAPK family that controls inflammation. We assumed that the mechanism of rutin in the repair of spinal cord injury is associated with the inhibition of p38 MAPK pathway. Allen’s method was used to establish a rat model of spinal cord injury. The rat model was intraperitoneally injected with rutin (30 mg/kg) for 3 days. After treatment with rutin, Basso, Beattie and Bresnahan locomotor function scores increased. Water content, tumor necrosis factor alpha, interleukin 1 beta, and interleukin 6 levels, p38 MAPK protein expression and caspase-3 and -9 activities in T8–9 spinal cord decreased. Oxidative stress related markers superoxide dismutase and glutathione peroxidase levels increased in peripheral blood. Rutin exerts neuroprotective effect through anti-oxidation, anti-inflammation, anti-apoptosis and inhibition of p38 MAPK pathway.

Key words: nerve regeneration, spinal cord injury, rutin, oxidative stress, antioxidant, anti-inflammation, p38 mitogen activated protein kinase pathway, anti-apoptosis, caspase-3, caspase-9, neural regeneration