中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (11): 1961-1967.doi: 10.4103/1673-5374.238715

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

微胶囊化许旺细胞移植抑制背根神经节P2X3受体介导的神经病理性疼痛

  

  • 收稿日期:2018-07-02 出版日期:2018-11-15 发布日期:2018-11-15
  • 基金资助:

    国家自然科学基金（81760418，81260190）；江西省自然科学基金（20132BAB205023，20151BAB205022）；江西省卫生厅科学技术项目资助项目（20173010）

 Microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in dorsal root ganglia and neuropathic pain

Ya-Ling Zhang1, De-Jian Chen2, Bao-Lin Yang1, Tao-Tao Liu3, Jia-Juan Li4, Xiu-Qi Wang5, Guo-Yong Xue1, Zeng-Xu Liu1   

  1. 1 Department of Anatomy, Basic Medical School, Nanchang University, Nanchang, Jiangxi Province, China
    2 First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China
    3 Fourth Clinical Medical College of Nanchang University, Nanchang, Jiangxi Province, China
    4 Second Clinical Medical College of Nanchang University, Nanchang, Jiangxi Province, China
    5 Queen Mary College of Nanchang University, Nanchang, Jiangxi Province, China
  • Received:2018-07-02 Online:2018-11-15 Published:2018-11-15
  • Contact: Zeng-Xu Liu, PhD,1965349157@qq.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81760418 and 81260190; the Natural Science Foundation of Jiangxi Province, No. 20132BAB205023, 20151BAB205022; a grant from Science and Technology Research Project of Jiangxi Education Department, No. GJJ13159; a grant from the Science and Technology Program of Department of Health of Jiangxi Province, No. 20173010.

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摘要：

已有研究表明，许旺细胞移植是促进神经修复最有前途的策略之一，在周围神经保护和髓鞘生成等方面具有重要作用，而微囊技术解决了细胞移植引起的免疫排斥反应。课题组前期研究表明微囊化嗅鞘细胞能减轻神经病理疼痛，为此作者假设微胶囊化许旺细胞也可抑制神经病理性疼痛。采用传代培养法培养大鼠许旺细胞，并对其进行微囊化处理；建立坐骨神经慢性压迫性损伤神经病理痛大鼠模型，分别采用许旺细胞及微囊化许旺细胞移植治疗，并设模型组和假手术组进行对比。于术前和术后1，3，5，7，9，11，14 d测定大鼠机械缩足反射阈值和热缩足反射的潜伏期。采用免疫荧光双标法于术后第14 d观察各组L4-5背根神经节P2X3受体的表达情况。结果显示，与模型组相比，采用许旺细胞及微囊化许旺细胞移植治疗的神经病理痛模型大鼠机械缩足反射阈值和热缩足反射潜伏期均明显升高，大鼠L4-5 背根神经节P2X3受体阳性细胞百分率明显降低，微囊化许旺细胞移植组上述指标变化最为明显。上述数据说明，微囊化许旺细胞移植对L4-5背根神经节P2X3受体介导的神经病理性疼痛有抑制作用。

orcid:0000-0002-8676-2342(Zeng-Xu Liu)

关键词: 神经病理痛, 周围神经损伤, 坐骨神经, 微囊化, 许旺细胞, P2X3受体, 背根神经节, 慢性压迫性损伤, 细胞移植, 神经修复, 神经再生

Abstract:

Schwann cell transplantation is a promising method to promote neural repair, and can be used for peripheral nerve protection and myelination. Microcapsule technology largely mitigates immune rejection of transplanted cells. We previously showed that microencapsulated olfactory ensheathing cells can reduce neuropathic pain and we hypothesized that microencapsulated Schwann cells can also inhibit neuropathic pain. Rat Schwann cells were cultured by subculture and then microencapsulated and were tested using a rat chronic constriction injury (CCI) neuropathic pain model. CCI rats were treated with Schwann cells or microencapsulated Schwann cells and were compared with sham and CCI groups. Mechanical withdrawal threshold and thermal withdrawal latency were assessed preoperatively and at 1, 3, 5, 7, 9, 11 and 14 days postoperatively. The expression of P2X3 receptors in L4–5 dorsal root ganglia of the different groups was detected by double-label immunofluorescence on day 14 after surgery. Compared with the chronic constriction injury group, mechanical withdrawal threshold and thermal withdrawal latency were higher, but the expression of P2X3 receptors was remarkably decreased in rats treated with Schwann cells and microencapsulated Schwann cells, especially in the rats transplanted with microencapsulated Schwann cells. The above data show that microencapsulated Schwann cell transplantation inhibits P2X3 receptor expression in L4–5 dorsal root ganglia and neuropathic pain.

Key words: nerve regeneration, neuropathic pain, peripheral nerve injury, sciatic nerve, microencapsulation, Schwann cells, P2X3 receptor, dorsal root ganglion, chronic constriction injury, cell transplantation, neural repair, neural regeneration