中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (12): 2164-2172.doi: 10.4103/1673-5374.262595

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

轴突断裂引起足底血管扩张可作为大鼠坐骨神经损伤后C类神经纤维传入功能的新型评估方法

  

  • 出版日期:2019-12-15 发布日期:2019-12-15
  • 基金资助:

    中国国家自然科学基金项目（81100939，81773713，81501610），江苏省政府资助项目（BRA2018223），江南大学公共卫生中心项目（JUPH201808），无锡市卫生计生委项目（MS201717），江苏省和南通市政府学术发展计划项目

Axonotmesis-evoked plantar vasodilatation as a novel assessment of C-fiber afferent function after sciatic nerve injury in rats

Xue-Song Wang 1 , Xue Chen 2 , Tian-Wen Gu 3 , Ya-Xian Wang 3 , Da-Guo Mi 4 , Wen Hu 3   

  1. 1 Department of Orthopedics, The Affiliated Hospital of Jiangnan University (The Third People’s Hospital of Wuxi City), Wuxi, Jiangsu Province, China
    2 Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu Province, China
    3 Key Laboratory for Neuroregeneration of Ministry of Education and Co-innovation Center for Neuroregeneration of Jiangsu Province, Nantong University, Nantong, Jiangsu Province, China
    4 Department of Orthopedics, Nantong Hospital of Traditional Chinese Medicine, Nantong, Jiangsu Province, China
  • Online:2019-12-15 Published:2019-12-15
  • Contact: Da-Guo Mi, MD, 2535213700@qq.com; Wen Hu, MD, PhD, hoo@ntu.edu.cn.
  • Supported by:

    This work was supported in part by the National Natural Science Foundation of China, No. 81100939 and 81773713 (to WH), No. 81501610 (to XC); the Research Project funded by Jiangsu Provincial Government of China, No. BRA2018223 (to DGM); the Public Health Center at Jiangnan University of China, No. JUPH201808 (to XSW); the Wuxi Commission of Public Health and Family Planning of China, No. MS201717 (to XSW); the Project of Academic Development Program by Governments of Jiangsu Province and Nantong City of China (to DGM).

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摘要：

神经功能恢复的定量评估，特别是感觉和自主神经功能的定量评估，是神经再生研究领域的一大挑战。作者既往研究发现血管舒缩活动的神经控制可能用于评估神经功能。因此实验首先建立了5种不同的大鼠左侧坐骨神经损伤修复模型：神经挤压伤、神经横断/缝合、神经缺损/自体移植修复、神经缺损/导管修复以及神经缺损/无再生，然后使用激光多普勒血流成像分析大鼠足底神经源性血管扩张程度，同时以趾捏反射试验和步迹分析评价大鼠后肢运动功能，以透射电镜观察损伤坐骨神经中无髓鞘轴突密度。结果发现不同类型神经损伤修复后，坐骨神经损伤引起足底皮肤血管扩张情况存在差异，且足底皮肤血管扩张的恢复指数与趾捏反射评分以及无髓神经纤维密度有相关性。由于C类神经纤维参与皮肤中浅表脉管系统的扩张，因此可将足底皮肤血管扩张情况作为周围神经损伤后C类神经纤维功能评价的一种新方法。实验于2013-4-10经南通大学实验动物伦理委员会批准，批准号20130410-006。

orcid: 0000-0003-0883-5566(Wen Hu)

关键词: 轴突损伤诱发的血管舒张, 激光多普勒血流成像, 神经功能, 自主神经, C类神经纤维传入功能, 周围神经损伤, 无髓传入纤维再生, 神经再生

Abstract:

Quantitative assessment of the recovery of nerve function, especially sensory and autonomic nerve function, remains a challenge in the field of nerve regeneration research. We previously found that neural control of vasomotor activity could be potentially harnessed to evaluate nerve function. In the present study, five different models of left sciatic nerve injury in rats were established: nerve crush injury, nerve transection/suturing, nerve defect/autografting, nerve defect/conduit repair, and nerve defect/non-regeneration. Laser Doppler perfusion imaging was used to analyze blood perfusion of the hind feet. The toe pinch test and walking track analysis were used to assess sensory and motor functions of the rat hind limb, respectively. Transmission electron microscopy was used to observe the density of unmyelinated axons in the injured sciatic nerve. Our results showed that axonotmesis-evoked vasodilatation in the foot 6 months after nerve injury/repair recovered to normal levels in the nerve crush injury group and partially in the other three repair groups; whereas the nerve defect/non-regeneration group exhibited no recovery in vasodilatation. Furthermore, the recovery index of axonotmesis-evoked vasodilatation was positively correlated with toe pinch reflex scores and the density of unmyelinated nerve fibers in the regenerated nerve. As C-fiber afferents are predominantly responsible for dilatation of the superficial vasculature in the glabrous skin in rats, the present findings indicate that axonotmesis-evoked vasodilatation can be used as a novel way to assess C-afferent function recovery after peripheral nerve injury. This study was approved by the Ethics Committee for Laboratory Animals of Nantong University of China (approval No. 20130410-006) on April 10, 2013.

Key words: nerve regeneration, axonotmesis-evoked vasodilatation, laser Doppler perfusion imaging, nerve function, autonomic nerve, C-fiber afferent function, peripheral nerve injury, unmyelinated afferent fiber regeneration, neural regeneration