中国神经再生研究(英文版) ›› 2020, Vol. 15 ›› Issue (1): 145-151.doi: 10.4103/1673-5374.264461

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

高频重复磁刺激联合神经妥乐平对大鼠损伤坐骨神经再生的影响

  

  • 出版日期:2020-01-15 发布日期:2020-05-20
  • 基金资助:
    国家自然科学基金(81471338,81671284);中国常州科技计划项目编号(CE20145045);中国常州高水平医学人才培养项目(2016CZLJ018)

Effect of the combination of high-frequency repetitive magnetic stimulation and neurotropin on injured sciatic nerve regeneration in rats

Jie Chen1, Xian-Ju Zhou2, 3, Rong-Bin Sun1   

  1. 1 Department of Orthopedics, the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, China
    2 Laboratory of Neurological Diseases, Department of Neurology, the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, Changzhou, Jiangsu Province, China
    3 Department of Neurology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong Province, China
  • Online:2020-01-15 Published:2020-05-20
  • Contact: Xian-Ju Zhou, PhD,xianju_zhou@yahoo.com; Rong-Bin Sun, MS,rongbin_sun@sina.com.
  • Supported by:
    This study was funded by the National Natural Science Foundation of China, No. 81471338 and No. 81671284 (to XJZ); the
    Changzhou Science & Technology Program of China, No. CE20145045 (to XJZ); the Changzhou High-Level Medical Talents Training Project of China, No. 2016CZLJ018 (to XJZ).

摘要:

重复磁刺激治疗已经被证明在治疗脊髓或轴突损伤后的神经病变是有效的,并且神经妥乐平也是一种治疗脱髓鞘神经损伤的有效药物。为此,实验拟观察高频重复磁刺激及神经妥乐平单独或两者联合治疗对周围神经损伤的影响。(1)实验切下大鼠左侧坐骨神经中10 mm的神经段后翻转缝合,然后将大鼠随机分为4组:对照组仅接受自体移植手术不接受任何治疗,高频重复磁刺激组术后给予10 d连续的外周高频重复磁刺激(20 Hz,20 min/d),神经妥乐平组术后给予10 d连续的神经妥乐平腹腔注射(0.96 NU/kg/d),联合治疗组采用上述两种方法联合治疗;(2)采用BBB运动评定量表进行评估大鼠行为学变化,测量并计算坐骨神经功能指数评估运动功能恢复情况,采用甲苯胺蓝染色法检测损伤神经远端和近端移植物有髓纤维的数目,采用免疫组织化学染色检测NF200标记的阳性轴突的总长度;(3)结果发现,3个治疗组的BBB评分、坐骨神经功能指数、近端和远端的有髓纤维计数总数、中间部分轴突计数总长度均高于对照组,但3个治疗组之间在上述指标变化上无显著差异;(4)上述数据说明,在大鼠坐骨神经损伤模型中,外周高频重复磁刺激和神经妥乐平药物治疗均可促进损伤坐骨神经修复,但两者联合治疗并没未见更好的修复效果。实验经南京医科大学附属常州市第二人民医院动物伦理委员会于2014年12月23日批准了本研究(批准号:2014keyan002-01)。

orcid: 0000-0003-1744-556X (Xian-Ju Zhou)
         0000-0003-2900-7276 (Rong-Bin Sun)

关键词: 神经再生, 重复磁刺激, 神经妥乐平, 周围神经损伤, 坐骨神经, 有髓神经纤维, 轴突, 神经康复

Abstract: Repetitive magnetic stimulation is effective for treating posttraumatic neuropathies following spinal or axonal injury. Neurotropin is a potential treatment for nerve injuries like demyelinating diseases. This study sought to observe the effects of high-frequency repetitive magnetic stimulation, neurotropin and their combined use in the treatment of peripheral nerve injury in 32 adult male Sprague-Dawley rats. To create a sciatic nerve injury model, a 10 mm-nerve segment of the left sciatic nerve was cut and rotated through 180° and each end restored continuously with interrupted sutures. The rats were randomly divided into four groups. The control group received only a reversed autograft in the left sciatic nerve with no treatment. In the high-frequency repetitive magnetic stimulation group, peripheral high-frequency repetitive magnetic stimulation treatment (20 Hz, 20 min/d) was delivered for 10 consecutive days after auto-grafting. In the neurotropin group, neurotropin therapy (0.96 NU/kg per day) was administrated for 10 consecutive days after surgery. In the combined group, the combination of peripheral high-frequency repetitive magnetic stimulation (20 Hz, 20 min/d) and neurotropin (0.96 NU/kg per day) was given for 10 consecutive days after the operation. The Basso-Beattie-Bresnahan locomotor rating scale was used to assess the behavioral recovery of the injured nerve. The sciatic functional index was used to evaluate the recovery of motor functions. Toluidine blue staining was performed to determine the number of myelinated fibers in the distal and proximal grafts. Immunohistochemistry staining was used to detect the length of axons marked by neurofilament 200. Our results reveal that the Basso-Beattie-Bresnahan locomotor rating scale scores, sciatic functional index, the number of myelinated fibers in distal and proximal grafts were higher and axon lengths were longer in the high-frequency repetitive magnetic stimulation, neurotropin and combined groups compared with the control group. These measures were not significantly different among the high-frequency repetitive magnetic stimulation, neurotropin and combined groups. Therefore, our results suggest that peripheral high-frequency repetitive magnetic stimulation or neurotropin can promote the repair of injured sciatic nerves, but their combined use seems to offer no significant advantage. This study was approved by the Animal Ethics Committee of the Affiliated Changzhou No. 2 People’s Hospital of Nanjing Medical University, China on December 23, 2014 (approval No. 2014keyan002-01).

Key words: axon, myelinated nerve fibers, nerve regeneration, neurological rehabilitation, neurotropin, peripheral nerve injury, repetitive magnetic stimulation, sciatic nerve, trauma