中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (9): 1617-1625.doi: 10.4103/1673-5374.255997

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

聚ε- 己内酯/I型胶原蛋白复合新型静电纺丝神经导管修复损伤周围神经

  

  • 出版日期:2019-09-15 发布日期:2019-09-15
  • 基金资助:

    中国台中退伍军人总医院和中央台湾科技大学和台中退伍军人总医院资助

Novel electrospun poly(ε-caprolactone)/type I collagen nanofiber conduits for repair of peripheral nerve injury

Chun-Ming Yen 1, 2 , Chiung-Chyi Shen 1, 3, 4 , Yi-Chin Yang 1 , Bai-Shuan Liu 5 , Hsu-Tung Lee 1 , Meei-Ling Sheu 6, 7, 8 , Meng-Hsiun Tsai 9 , Wen-Yu Cheng 1, 3   

  1. 1 Department of Neurosurgery, Neurological Institute, Taichung Veterans General Hospital, Taichung, Taiwan, China
    2 Ph.D. Program in Translational Medicine, National Chung Hsing University, Taichung, Taiwan, China
    3 Department of Physical Therapy, Hungkuang University, Taichung, Taiwan, China
    4 Basic Medical Education Center, Central Taiwan University of Science and Technology, Taichung, Taiwan, China
    5 Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung, Taiwan, China
    6 Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan, China
    7 Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan, China
    8 Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung, Taiwan, China
    9 Department of Management Information System, National Chung Hsing University, Taichung, Taiwan, China
  • Online:2019-09-15 Published:2019-09-15
  • Contact: Wen-Yu Cheng, PhD, wycheng07@yahoo.com.tw.
  • Supported by:

    This study was supported by grants from the Taichung Veterans General Hospital and Central Taiwan University of Science and Technology, No. TCVGH-CTUST1047701 (to CCS and BSL) and Taichung Veterans General Hospital, No. TCVGH-1034907C (to CCS), Taiwan, China.

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摘要：

已有研究显示出了人工合成神经导管在周围神经损伤后修复中的应用潜力，其中生物相容性好的天然生物聚合物更是引起广泛关注。为观察可吸收的聚ε- 己内酯/I型胶原蛋白复合新型静电纺丝神经导管修复周围神经损伤的效果，实验以聚ε- 己内酯/I型胶原蛋白复合静电纺丝纳米纤维神经导管或聚ε- 己内酯神经导管或硅胶修复SD大鼠10mm坐骨神经缺损。修复后的8周内，每周以坐骨神经指数评估神经功能。修复后8周，以锇酸染色、HE染色和透射电镜观察坐骨神经髓鞘和轴突形态，免疫组化染色检测坐骨神经反映许旺细胞标志物S-100和炎症反应标志物CD4免疫反应。结果显示，聚ε- 己内酯/I型胶原蛋白复合静电纺丝神经导管的大鼠后肢中未见严重的炎症反应，其损伤坐骨神经髓鞘形态接受正常；CD4免疫阳性反应明显较聚ε- 己内酯神经导管或硅胶修复组弱；聚ε- 己内酯/I型胶原蛋白复合静电纺丝神经导管修复组大鼠坐骨神经功能恢复较其他组显示出更好的趋势。说明生物可吸收生物材料聚ε- 己内酯/I型胶原蛋白复合静电纺丝神经导管具有修复坐骨神经缺损的潜力，且生物相容性较好。

orcid: 0000-0003-4661-3809 (Wen-Yu Cheng)

关键词: 聚己内酯, I型胶原蛋白, 静电纺丝, 坐骨神经, 神经导管, 免疫组织化学染色, 足迹分析, 周围神经损伤

Abstract:

Recent studies have shown the potential of artificially synthesized conduits in the repair of peripheral nerve injury. Natural biopolymers have received much attention because of their biocompatibility. To investigate the effects of novel electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits (biopolymer nanofiber conduits) on the repair of peripheral nerve injury, we bridged 10-mm-long sciatic nerve defects with electrospun absorbable biopolymer nanofiber conduits, poly(ε-caprolactone) or silicone conduits in Sprague-Dawley rats. Rat neurologica1 function was weekly evaluated using sciatic function index within 8 weeks after repair. Eight weeks after repair, sciatic nerve myelin sheaths and axon morphology were observed by osmium tetroxide staining, hematoxylin-eosin staining, and transmission electron microscopy. S-100 (Schwann cell marker) and CD4 (inflammatory marker) immunoreactivities in sciatic nerve were detected by immunohistochemistry. In rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits, no serious inflammatory reactions were observed in rat hind limbs, the morphology of myelin sheaths in the injured sciatic nerve was close to normal. CD4 immunoreactivity was obviously weaker in rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits than in those subjected to repair with poly(ε-caprolactone) or silicone. Rats subjected to repair with electrospun absorbable biopolymer nanofiber conduits tended to have greater sciatic nerve function recovery than those receiving poly(ε-caprolactone) or silicone repair. These results suggest that electrospun absorbable poly(ε-caprolactone)/type I collagen nanofiber conduits have the potential of repairing sciatic nerve defects and exhibit good biocompatibility. All experimental procedures were approved by Institutional Animal Care and Use Committee of Taichung Veteran General Hospital, Taiwan, China (La-1031218) on October 2, 2014.

Key words: poly(ε-caprolactone), type I collagen, electrospinning, sciatic nerve, nerve conduit, immunohistostaining, walking track analysis, peripheral nerve injury