中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (9): 2005-2010.doi: 10.4103/1673-5374.366491

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

光生物调节对脊髓损伤的神经保护:调节亚急性期线粒体动力学失衡

  

  • 出版日期:2023-09-15 发布日期:2023-03-06
  • 基金资助:
    国家自然科学基金项目(81070996,81572151),陕西省重点研发计划项目(2020ZDLSF02-05,2021ZDLSF02-10)

Photobiomodulation provides neuroprotection through regulating mitochondrial fission imbalance in the subacute phase of spinal cord injury

Xin Li1, 2, #, Xuan-Kang Wang1, #, Zhi-Jie Zhu1, #, Zhuo-Wen Liang1, Peng-Hui Li1, Yang-Guang Ma1, Tan Ding1, Kun Li1, Xiao-Shuang Zuo1, Cheng Ju1, Zhi-Hao Zhang1, Zhi-Wen Song1, Hui-Lin Quan1, Jia-Wei Zhang1, Liang Luo1, Zhe Wang1, *, Xue-Yu Hu1, *   

  1. 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi Province, China; 2967 Hospital of People’s Liberation Army Joint Logistic Support Force, Dalian, Liaoning Province, China
  • Online:2023-09-15 Published:2023-03-06
  • Contact: Xue-Yu Hu, MD, huxueyu@fmmu.edu.cn; Zhe Wang, MD, wangzhe@fmmu.edu.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81070996 (to ZW) and  81572151 (to XYH); and Shaanxi Provincial Key R&D Program, Nos. 2020ZDLSF02-05 (to ZW), 2021ZDLSF02-10 (to XYH).

摘要:

越来越多的证据表明,线粒体动力学失衡在脊髓损伤继发性损伤病理过程中起着至关重要的作用。作者既往研究已发现光生物调节可有效改善脊髓损伤大鼠的运动功能,但其具体机制并未明确。为探索光生物调节对脊髓损伤后线粒体动力学的影响,实验连续14d,每天60min的光生物调节(810 nm,150 mW)对脊髓损伤大鼠模型进行治疗,透射电镜可见在脊髓损伤急性期(1d)和亚急性期(7和14d),大鼠脊髓神经元线粒体中存在肿胀和碎片化的迹象,而光生物调节可在亚急性期显著缓解脊髓组织中线粒体动力学失衡,并减轻神经元死亡,改善大鼠的后肢运动功能,且这一作用存在时间积累性。结果提示光生物调节直接靶向神经元内的线粒体,缓解线粒体动力学失衡诱导的神经元凋亡,进而促进脊髓损伤大鼠运动功能的恢复。

https://orcid.org/0000-0003-0852-1196 (Xue-Yu Hu); https://orcid.org/0000-0002-7573-1583 (Zhe Wang)

关键词: 光生物调节, 线粒体动力学, 脊髓损伤, 挫伤, 迟发性继发性损伤, 神经元, 线粒体, 裂变, 线粒体动力学失衡, 低强度激光治疗

Abstract: Increasing evidence indicates that mitochondrial fission imbalance plays an important role in delayed neuronal cell death. Our previous study found that photobiomodulation improved the motor function of rats with spinal cord injury. However, the precise mechanism remains unclear. To investigate the effect of photobiomodulation on mitochondrial fission imbalance after spinal cord injury, in this study, we treated rat models of spinal cord injury with 60-minute photobiomodulation (810 nm, 150 mW) every day for 14 consecutive days. Transmission electron microscopy results confirmed the swollen and fragmented alterations of mitochondrial morphology in neurons in acute (1 day) and subacute (7 and 14 days) phases. Photobiomodulation alleviated mitochondrial fission imbalance in spinal cord tissue in the subacute phase, reduced neuronal cell death, and improved rat posterior limb motor function in a time-dependent manner. These findings suggest that photobiomodulation targets neuronal mitochondria, alleviates mitochondrial fission imbalance-induced neuronal apoptosis, and thereby promotes the motor function recovery of rats with spinal cord injury. 

Key words: low-level laser therapy, mitochondria, mitochondrial dynamics, mitochondrial fission imbalance, neuron, photobiomodulation, secondary injury, spinal cord injury