中国神经再生研究(英文版) ›› 2026, Vol. 21 ›› Issue (10): 4945-4953.doi: 10.4103/NRR.NRR-D-25-00491

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

小鼠皮质及皮质-纹状体卒中的动态结构连接

  

  • 出版日期:2026-10-15 发布日期:2026-06-13

Dynamic structural connectivity changes in cortical and cortico-striatal strokes in mice

Fatemeh Mahani1, 2, Aref Kalantari2, Michael Diedenhofen3, Claudia Green3, Dirk Wiedermann3, Gereon R. Fink1, 2, Mathias Hoehn1, Markus Aswendt2, 4, *   

  1. 1Cognitive Neuroscience, Institute of Neuroscience and Medicine (INM-3), Research Centre Juelich, Juelich, Germany; 
    2University of Cologne, Faculty of Medicine and University Hospital Cologne, Department of Neurology, Cologne, Germany; 
    3In-vivo-NMR Laboratory, Max Planck Institute for Metabolism Research, Cologne, Germany; 
    4Goethe University Frankfurt and University Hospital, Department of Neurology, Experimental Neurology Section, Frankfurt am Main, Germany
  • Online:2026-10-15 Published:2026-06-13
  • Contact: Markus Aswendt, PhD, aswendt@med.uni-frankfurt.de.
  • Supported by:
    This work was supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) — SFB 1451 — Project-ID 431549029 (to GRFand MA).

摘要:

脑卒中除即时神经元损伤外,全脑功能与结构连接性均发生改变,进而影响功能缺损及康复进程。然而,轴突损伤程度及代偿性可塑性(即轴突萌发与髓鞘再生)是否取决于病灶大小与拓扑结构仍不明确。实验首次对比成年雄性小鼠两种不同卒中模型,旨在揭示白质变化的动态机制。在光诱血栓性皮质卒中(占脑体积1.41±0.92%)及中大脑动脉闭塞(MCAO)皮质-纹状体卒中(占脑体积11.53±2.8%)后4周内,采用重复扩散磁共振成像(dMRI)进行采集。通过全脑层面映射结构连接性随时间的变化。量化了半球间与半球内种子强度随时间的变化,其中种子强度反映各区域与大脑其他区域的连接强度。采用经多重比较校正的混合模型评估组间及时间点间的差异。结果表明,大范围皮质-纹状体损伤导致感觉运动区结构连接性增强;而小范围皮质损伤则引发不对称连接变化——缺血半球呈现全球性连接增强,健康半球呈现全球性连接减弱。这些发现揭示脑卒中严重程度与病灶大小显著影响连接性破坏的时间动态与空间分布,强调了脑卒中后神经变化需进行靶向监测的必要性。


https://orcid.org/0000-0003-1423-0934 (Markus Aswendt)

关键词: 脑网络, 纤维追踪, 大脑中动脉阻塞闭, 变性, 白质可塑性, 小鼠, 纵向研究, 啮齿动物

Abstract: Beyond immediate neuronal damage, functional and structural connectivity is altered brain-wide with implications for functional deficits and recovery in stroke. It remains unclear, however, if the level of axonal damage, as well as compensatory plasticity, i.e., axonal sprouting and remyelination, depend on the lesion size and topology. This study compared two different stroke models in adult male mice, with the aim of uncovering the dynamics in white matter changes. Repetitive diffusion magnetic resonance imaging was acquired over 4 weeks post photothrombotic cortical (1.41% ± 0.92% of brain volume) and middle cerebral artery occlusion cortico-striatal (11.53% ± 2.8% of brain volume) strokes. Structural connectivity changes were mapped over time at the whole-brain level. We quantified inter- and intra-hemispheric seed strength changes over time, with seed strength reflecting how strongly each region was connected to the rest of the brain. Differences between groups and time points were assessed using a mixed model corrected for multiple comparisons. The results showed that large cortico-striatal lesions led to increased structural connectivity in sensorimotor regions, whereas small cortical lesions induced asymmetric connectivity changes: an increase extending globally from the ischemic hemisphere and a decrease expanding globally from the healthy hemisphere. These findings highlight that stroke severity and lesion size significantly affect the temporal dynamics and spatial distribution of connectivity disruptions, emphasizing the need for targeted monitoring of neural changes post-stroke.

Key words: brain network, degeneration, diffusion MRI, fiber tracking, longitudinal, middle cerebral artery occlusion, mouse, photothrombosis, rodent, white matter plasticity