中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2050-2059.doi: 10.3969/j.issn.1673-5374.2013.22.004

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

白藜芦醇干预血管性痴呆大鼠认知及氧化损伤的变化

  

  • 收稿日期:2013-04-15 修回日期:2013-06-02 出版日期:2013-08-05 发布日期:2013-08-05

Resveratrol improves cognition and reduces oxidative stress in rats with vascular dementia

Xingrong Ma1, Zhikun Sun2, Yanru Liu1, Yanjie Jia1, Boai Zhang1, Jiewen Zhang2   

  1. 1 Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China
    2 Department of Neurology, Henan Provincial People’s Hospital, Zhengzhou 450002, Henan Province, China
  • Received:2013-04-15 Revised:2013-06-02 Online:2013-08-05 Published:2013-08-05
  • Contact: Boai Zhang, M.D., Chief physician, Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China, zhangboaidoctor@ 163.com; Jiewen Zhang, M.D., Chief physician, Department of Neurology, Henan Provincial People’s Hospital, Zhengzhou 450002, Henan Province, China, zhangjiewen9900@126.com.
  • About author:Xingrong Ma, Master, Attending physician. Xingrong Ma and Zhikun Sun contributed equally to this article.

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摘要：

白藜芦醇具有抗氧化、抗炎等生物学效应，对脑缺血、帕金森病和阿尔茨海默病有神经保护作用，然而，其对血管性痴呆的作用尚不明确。实验采用永久性夹闭双侧颈总动脉方法制备大鼠血管性痴呆模型，造模后8-12周，每天向模型大鼠灌胃25mg/kg白藜芦醇。结果发现，白藜芦醇可缩短Morris水迷宫训练过程中模型大鼠的逃避潜伏期和游泳距离，延长模型大鼠在空间探索实验中的目标象限的停留时间和游泳距离百分比。说明白藜芦醇对血管性痴呆大鼠的学习记忆能力有提高作用。进一步检测发现，白藜芦醇可抑制模型大鼠脑皮质和海马中丙二醛水平的增高及超氧化物歧化酶活性和谷胱甘肽含量的降低。提示白藜芦醇对血管性痴呆的神经保护作用与抗氧化有关。

关键词: 神经再生, 中医药, 白藜芦醇, 血管性痴呆, 认知功能, 学习记忆, 氧化应激, 双侧颈总动脉夹闭, 丙二醛, 超氧化物歧化酶

Abstract:

Resveratrol possesses beneficial biological effects, which include anti-oxidant, anti-inflammatory and anti-carcinogenic properties. Recently, resveratrol has been shown to exhibit neuroprotective effects in models of Parkinson’s disease, cerebral ischemia and Alzheimer’s disease. However, its effects on vascular dementia remain unclear. The present study established a rat model of vascular dementia using permanent bilateral common carotid artery occlusion. At 8–12 weeks after model induction, rats were intragastrically administered 25 mg/kg resveratrol daily. Our results found that resveratrol shortened the escape latency and escape distances in the Morris water maze, and pro-longed the time spent percentage and swimming distance percentage in the target quadrant during the probe test, indicating that resveratrol improved learning and memory ability in vascular dementia rats. Further experiments found that resveratrol decreased malonyldialdehyde levels, and increased superoxide dismutase activity and glutathione levels in the hippocampus and cerebral cortex of vascular dementia rats. These results confirmed that the neuroprotective effects of resveratrol on vascular dementia were associated with its anti-oxidant properties.