中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (22): 2093-2102.doi: 10.3969/j.issn.1673-5374.2013.22.009

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

人脐带间充质干细胞体内向神经胶质瘤迁移的能力

  

  • 收稿日期:2013-04-25 修回日期:2013-06-30 出版日期:2013-08-05 发布日期:2013-08-05

Migration capacity of human umbilical cord mesenchymal stem cells towards glioma in vivo

Cungang Fan, Dongliang Wang, Qingjun Zhang, Jingru Zhou   

  1. Department of Neurosurgery, Peking University People’s Hospital, Beijing 100044, China
  • Received:2013-04-25 Revised:2013-06-30 Online:2013-08-05 Published:2013-08-05
  • Contact: Qingjun Zhang, Chief physician, Professor, Doctoral supervisor, Department of Neurosurgery, Peking University People’s Hospital, Beijing 100044, China, zhangqjhb@ yahoo.com.
  • About author:Cungang Fan, Master, Attending physician, Principal investigator.
  • Supported by:

    国家自然科学基金(No.81001009)。

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摘要：

脑胶质瘤发病早期即向周围正常脑组织内浸润生长而在瘤床周围形成的卫星灶，为此迫切需要研究能够追踪此类肿瘤细胞浸润的靶向治疗策略，干细胞作为一种新的基因靶向治疗的运载媒介正逐渐受到重视。在研究中，我们分离培养的人脐带间充质干细胞显示出塑料粘附的特点，在流式细胞分析中表达间充质干细胞的特殊表型，可在体外诱导表现出多向分化的潜能。脑胶质瘤大鼠模型瘤内或对侧脑室内注射人脐带间充质干细胞，发现人脐带间充质干细胞能表现出良好的胶质瘤特异性靶向，且在瘤内广泛分布，表明人脐带间充质干细胞可能作为一种新型的细胞载体用于脑胶质瘤的靶向治疗。

关键词: 神经再生, 干细胞, 间充质干细胞, 脐带, 胶质瘤, 迁移, 趋向性, 细胞载体, 基金资助文章

Abstract:

High-grade glioma is the most common malignant primary brain tumor in adults. The poor prognosis of glioma, combined with a resistance to currently available treatments, necessitates the develop-ment of more effective tumor-selective therapies. Stem cell-based therapies are emerging as novel cell-based delivery vehicle for therapeutic agents. In the present study, we successfully isolated human umbilical cord mesenchymal stem cells by explant culture. The human umbilical cord mesenchymal stem cells were adherent to plastic surfaces, expressed specific surface phenotypes of mesenchymal stem cells as demonstrated by flow cytometry, and possessed multi-differentiation potentials in permissive induction media in vitro. Furthermore, human umbilical cord mesenchymal stem cells demonstrated excellent glioma-specific targeting capacity in established rat glioma models after intratumoral injection or contralateral ventricular administration in vivo. The excellent glioma-specific targeting ability and extensive intratumoral distribution of human umbilical cord mesenchymal stem cells indicate that they may serve as a novel cellular vehicle for delivering therapeutic molecules in glioma therapy.

Key words: neural regeneration, umbilical cord, mesenchymal stem cell, glioma, migration, cell-based therapy, grants-supported paper, neuroregeneration