中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (9): 931-936.doi: 10.4103/1673-5374.133135

• 原著:退行性病与再生 • 上一篇    下一篇

止颤汤诱导移植干细胞帕金森病大鼠中脑神经元向多巴胺能神经元分化

  

  • 收稿日期:2014-04-21 出版日期:2014-05-20 发布日期:2014-05-20
  • 基金资助:

    国家自然科学基金项目(30772870)资助

Zhichan decoction induces differentiation of dopaminergic neurons in Parkinson’s disease rats after neural stem cell transplantation

Huifen Shi 1, Jie Song 2, Xuming Yang 3   

  1. 1 Department of Neurology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai, China
    2 Department of Encephalopathy, Liu’an Hospital of Traditional Chinese Medicine, Liu’an, Anhui Province, China
    3 College of Acupuncture and Massage, Shanghai University of Traditional Chinese Medicine, Shanghai, China
  • Received:2014-04-21 Online:2014-05-20 Published:2014-05-20
  • Contact: Xuming Yang, College of Acupuncture and Massage, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China, fslbest@126.com.
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of    China, No. 30772870.

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摘要：

实验以促进脑内多巴胺含量增加以及减少多巴胺代谢为目标，用高效液相色谱法检测显示，神经干细胞移植+中药止颤汤灌胃干预的帕金森病模型大鼠中脑组织多巴胺及多巴胺能神经元的重要代谢物双羟苯乙酸和高香草酸含量均高于单纯神经干细胞移植组。由遗传算法求得双羟苯乙酸和高香草酸含量的全局最优解，发现神经干细胞移植+止颤汤灌胃能提高帕金森病模型大鼠中脑组织双羟苯乙酸含量10倍以上、单纯神经干细胞移植干预能提高3倍以上，而对高香草酸含量的提高则无显著影响。结果说明中药止颤汤对帕金森病模型大鼠移植后的神经干细胞分化为多巴胺能神经元有诱导作用。

关键词: 神经再生, 中医药, 神经退行性变, 帕金森病, 大鼠模型, 止颤汤, 干细胞移植, 多巴胺代谢, 双羟苯乙酸, 高香草酸, 曲线方程拟合, 遗传算法, 最优化模型, 国家自然科学基金

Abstract:

The goal of this study was to increase the dopamine content and reduce dopaminergic metabolites in the brain of Parkinson’s disease rats. Using high-performance liquid chromatography, we found that dopamine and dopaminergic metabolite (dihydroxyphenylacetic acid and homovanillic acid) content in the midbrain of Parkinson’s disease rats was increased after neural stem cell transplantation + Zhichan decoction, compared with neural stem cell transplantation alone. Our genetic algorithm results show that dihydroxyphenylacetic acid and homovanillic acid levels achieve global optimization. Neural stem cell transplantation + Zhichan decoction increased dihydroxyphenylacetic acid levels up to 10-fold, while transplantation alone resulted in a 3-fold increment. Homovanillic acid levels showed no apparent change. Our experimental findings show that after neural stem cell transplantation in Parkinson’s disease rats, Zhichan decoction can promote differentiation of neural stem cells into dopaminergic neurons.

Key words: nerve regeneration, traditional Chinese medicine, neurodegeneration, Parkinson’s disease, rat model, Zhichan decoction, stem cell transplantation, dopamine metabolite, dihydroxyphenylacetic acid, homovanillic acid, curve fitting equation, genetic algorithm, optimization model, NSFC grant, neural degeneration