中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (11): 1814-1818.doi: 10.4103/1673-5374.170309

• 原著:退行性病与再生 • 上一篇    下一篇

过表达Persephin基因转染大鼠黑质多巴胺神经细胞建立帕金森病模型

  

  • 收稿日期:2015-09-14 出版日期:2015-12-07 发布日期:2015-12-07
  • 基金资助:

    中国国家自然科学基金项目（81171208），山东省自然科学基金项目（Z2008C06

Lentivirus-mediated Persephin over-expression in Parkinson’s disease rats

Xiao-feng Yin1, #, Hua-min Xu2, #, Yun-xia Jiang3, Yun-lai Zhi4, Yu-xiu Liu5, Heng-wei Xiang6, Kai Liu6, Xiao-dong Ding6, *, Peng Sun6, *   

  1. 1 Department of Neurosurgery, the Second Affiliated Hospital of Shanxi Medical University, Taiyuan, Shanxi Province, China
    2 Department of Physiology, Qingdao University, Qingdao, Shandong Province, China
    3 Nursing College of Qingdao University, Qingdao, Shandong Province, China
    4 Department of Pediatric Surgery, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    5 Department of Nursing, Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, China
    6 Department of Neurosurgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao, Shandong Province, China
  • Received:2015-09-14 Online:2015-12-07 Published:2015-12-07
  • Contact: Peng Sun, M.D. or Xiao-dong Ding, M.D., sunpengqd@163.com or 15154258721@163.com.
  • About author:Peng Sun, M.D. or Xiao-dong Ding, M.D., sunpengqd@163.com or 15154258721@163.com.
  • Supported by:

    This work was supported by the National Natural Science Foundation of China, No. 81171208 and the Natural Science Foundation of Shandong Province of China, No. Z2008C06

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摘要：

Persephin能参与胶质细胞源性神经营养因子和Neurturin的神经营养作用，促进体外培养运动神经元的存活。实验以Persephin基因预先转染大鼠黑质多巴胺能神经细胞，然后再在内侧前脑束注射6-羟多巴胺建立帕金森病大鼠模型，发现其黑质中多巴胺能神经元数量增加，酪氨酸羟化酶表达水平上升，纹状体中多巴胺及其代谢产物的浓度增加，且在一定程度上改善了帕金森病模型大鼠的旋转行为。说明Persephin通过保护多巴胺能神经细胞对6-羟多巴胺诱导的帕金森病模型大鼠起到了治疗和神经保护作用。

关键词: 神经再生, Persephin, 慢病毒, 帕金森病, 多巴胺能神经细胞, 基因治疗, 过表达, 转染, 纹状体

Abstract:

Persephin, together with glial cell line-derived neurotrophic factor and neurturin, has a neurotrophic
effect and promotes the survival of motor neurons cultured in vitro. In this study, dopaminergic neurons in the substantia nigra of rats were transfected with the Persephin gene. One week later 6-hydroxydopamine was injected into the anterior medial bundle to establish a Parkinson’s
disease model in the rats. Results found that the number of dopaminergic neurons in the substantia nigra increased, tyrosine hydroxylase expression was upregulated and concentrations of dopamine and its metabolites in corpus striatum were increased after pretreatment with Persephin gene. In addition, the rotating effect of the induced Parkinson’s disease rats was much less in the group pretreated with the Persephin gene. Persephin has a neuroprotective effect on the 6-hydroxydopamine-induced Parkinson’s disease through protecting dopaminergic neurons.

Key words: nerve regeneration, Persephin, lentivirus, Parkinson’s disease, dopaminergic neurons, gene therapy, over-expression, transfection, striatum, neural regeneration