中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (3): 508-511.doi: 10.4103/1673-5374.179075

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

维拉帕米抑制周围神经损伤修复后的瘢痕形成

  

  • 收稿日期:2015-11-18 出版日期:2016-03-15 发布日期:2016-03-15
  • 基金资助:
    湖北省自然科学基金(2011CDB392)
     

Verapamil inhibits scar formation after peripheral nerve repair in vivo

A-chao Han 1, Jing-xiu Deng 2, Qi-shun Huang 1, Huai-yuan Zheng 1, Pan Zhou 1, Zhi-wei Liu 1, Zhen-bing Chen 1   

  1. 1 Department of Hand Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
    2 457 Hospital of PLA, Wuhan, Hubei Province, China
  • Received:2015-11-18 Online:2016-03-15 Published:2016-03-15
  • Contact: Qi-shun Huang, M.D.,hqsw@hotmail.com.
  • Supported by:

    This research was supported by the Natural Science Foundation of Hubei Province of China, No. 2011CDB392.

摘要:

钙离子通道阻滞剂维拉帕米可以在体外抑制通过抑制成纤维细胞的粘附和增殖,减少瘢痕的形成,但在体内神经断裂修复后局部应用,能否也能抑制局部过多的瘢痕组织形成目前尚不清楚。为此,实验切断成年SD大鼠右侧坐骨神经,以10-0号显微缝线进行神经外膜缝合,将吸附维拉帕米溶液的吸收性明胶海绵包裹在神经吻合口周围。4周后,与神经吻合口周围包裹吸附生理盐水的吸收性明胶海绵的大鼠相比,维拉帕米明显抑制坐骨神经中成纤维细胞的生长及分泌细胞外基质,抑制Ⅰ和 III型胶原的分泌,增加了神经轴突总数和有髓轴突的数量,说明维拉帕米在体内环境下确有减少周围神经损伤修复后瘢痕组织的形成,促进神经轴突生长的作用。

关键词: 神经再生, 神经损伤, 维拉帕米, 瘢痕, 坐骨神经, Ⅰ型胶原蛋白, III型胶原蛋白

Abstract:

The calcium channel blocker, verapamil, has been shown to reduce scar formation by inhibiting fibroblast adhesion and proliferation in vitro. It was not clear whether topical application of verapamil after surgical repair of the nerve in vivo could inhibit the formation of excessive scar tissue. In this study, the right sciatic nerve of adult Sprague-Dawley rats was transected and sutured with No. 10-0 suture. The stoma was wrapped with gelfoam soaked with verapamil solution for 4 weeks. Compared with the control group (stoma wrapped with gelfoam soaked with physiological saline), the verapamil application inhibited the secretion of extracellular matrix from fibroblasts in vivo, suppressed type I and III collagen secretion and increased the total number of axons and the number of myelinated axons. These findings suggest that verapamil could reduce the formation of scar tissue and promote axon growth after peripheral nerve repair.

Key words: nerve regeneration, nerve injury, verapamil, scar, sciatic nerve, type I collagen, type III collagen, neural regeneration