中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (8): 1606-1612.doi: 10.4103/1673-5374.303041

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

蝶翅应用于临床组织工程化神经移植材料的生物相容性和安全性

  

  • 出版日期:2021-08-15 发布日期:2021-01-13
  • 基金资助:

    国家自然科学基金项目(31971276);江苏省高等学校自然科学基金(重大项目)(19KJA320005)

Biocompatibility and biosafety of butterfly wings for the clinical use of tissue-engineered nerve grafts

Shu Wang1, Miao Gu2, Cheng-Cheng Luan1, Yu Wang1, Xiaosong Gu1, Jiang-Hong He1, *   

  1. 1Key Laboratory for Neuroregeneration of Jiangsu Province and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China; 2Department of Basic Medicine, Chengde Medical College, Chengde, Hebei Province, China
  • Online:2021-08-15 Published:2021-01-13
  • Contact: Jiang-Hong He,PhD, hejh@ntu.edu.cn.
  • Supported by:
    This work was supported by the National Natural Science Foundation of China, No. 31971276, and the Natural Science Foundation of Jiangsu Higher Education Institutions of China (Major Program), No. 19KJA320005 (both to JHH).

摘要:

作者既往将天然蝶翅作为细胞生长基质和组织工程材料使用时,发现不同种类蝴蝶的翅膀的表面具有不同超微结构,可影响细胞的定性生长并调节细胞的生长代谢及基因表达,但其生物相容性和生物安全性还有待研究。此次实验发现来源于SD大鼠的背根神经节背根神经节神经元可沿着蝶翅结构条纹生长,且来源于SD大鼠坐骨神经的许旺细胞可在不同种属的蝶翅表面正常黏附并增殖。通过皮下注射SD大鼠、腹腔注射ICR小鼠、注射兔以及外敷豚鼠进一步检测蝶翅的生物相容性和安全性。结果显示蝶翅均无毒性作用,动物表现正常,无红斑水肿等症状。表明蝶翅具有优良的生物相容性和生物安全性,是一种良好的组织工程材料。实验于2019年3月3日经江苏省实验动物伦理委员会批准,批准号20190303-18。

https://orcid.org/0000-0002-5185-7982 (Jiang-Hong He)

关键词: 蝶翅, 许旺细胞, 背根神经节, 神经元, 生物材料, 生物相容性, 生物安全性, 组织工程

Abstract: In a previous study, we used natural butterfly wings as a cell growth matrix for tissue engineering materials and found that the surface of different butterfly wings had different ultramicrostructures, which can affect the qualitative growth of cells and regulate cell growth, metabolism, and gene expression. However, the biocompatibility and biosafety of butterfly wings must be studied. In this study, we found that Sprague-Dawley rat dorsal root ganglion neurons could grow along the structural stripes of butterfly wings, and Schwann cells could normally attach to and proliferate on different species of butterfly wings. The biocompatibility and biosafety of butterfly wings were further examined through subcutaneous implantation in Sprague-Dawley rats, intraperitoneal injection in Institute of Cancer Research mice, intradermal injection in rabbits, and external application to guinea pigs. Our results showed that butterfly wings did not induce toxicity, and all examined animals exhibited normal behaviors and no symptoms, such as erythema or edema. These findings suggested that butterfly wings possess excellent biocompatibility and biosafety and can be used as a type of tissue engineering material. This study was approved by the Experimental Animal Ethics Committee of Jiangsu Province of China (approval No. 20190303-18) on March 3, 2019.

Key words: biocompatibility, biomaterials, biosafety, butterfly wings, dorsal root ganglion, neurons, Schwann cells, tissue engineering