中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (1): 170-175.doi: 10.4103/1673-5374.344838

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

脑干出血的早期损伤进展:多序列磁共振成像和组织病理学观察

  

  • 出版日期:2023-01-15 发布日期:2022-06-17
  • 基金资助:
    新疆维吾尔自治区自然科学基金项目(2020D01A13);成都市科技局项目(2019-YF05-00511-SN);1.3.5四川大学华西医院卓越学科项目(ZY2016102,ZY2016203)

Early brainstem hemorrhage progression: multi-sequence magnetic resonance imaging and histopathology

Xi Guo1, #, Jia-Ke Xu1, #, Xin Qi1, Yang Wei1, Cheng-Wei Wang2, 3, Hao Li1, Lu Ma1, Chao You1, 4, Meng Tian1, 4, *   

  1. 1Department of Neurosurgery and Neurosurgery Research Laboratory, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China; 2Department of Integrated Traditional and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China; 3Department of Rehabilitation, People’s Hospital of Karamay, Karamay, Xinjiang Uygur Autonomous Region, China; 4West China Brain Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan Province, China
  • Online:2023-01-15 Published:2022-06-17
  • Contact: Meng Tian, PhD, tianmeng@scu.edu.cn.
  • Supported by:
    This study was supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2020D01A13 (to CWW); Chengdu Science and Technology Bureau, No. 2019-YF05-00511-SN (to MT); and 1.3.5 Project for Disciplines of Excellence, West China Hospital, Sichuan University, Nos. ZY2016102 (to MT), and ZY2016203 (to CY). 

摘要:

临床统计脑干出血患者的早期死亡率较高。为此,实验建立右侧基底节桥注射VII型胶原酶的脑出血模型大鼠,以多序列磁共振成像和组织病理学方法研究其早期病理变化。发现损伤大鼠的脑干血肿主要在前3d逐渐形成,而7d后减少,其血肿主要为血管源性水肿。同时在脑干出血病灶部位周围未见完整的髓鞘结构,神经纤维的完整性和连续性在7d内逐渐恶化;且神经元变性在前3d轻微,而在第7天明显加重。血肿周围组织中炎性细胞因子白细胞介素和肿瘤坏死因子α在出血后第1天产生,第3天达到高峰,到第7天下降。这些结果显示了脑干出血早期进展的变化特征。

https://orcid.org/0000-0002-6445-7670 (Meng Tian)

关键词:

脑干出血, 大鼠模型, T2加权成像, 弥散加权成像, 弥散张量成像, 苏木精-伊红染色, Luxol固蓝染色, Fluoro-Jade C染色, 白细胞介素, 肿瘤坏死因子α

Abstract: According to clinical statistics, the mortality of patients with early brainstem hemorrhage is high. In this study, we established rat models of brainstem hemorrhage by injecting type VII collagenase into the right basotegmental pontine and investigated the pathological changes of early brainstem hemorrhage using multi-sequence magnetic resonance imaging and histopathological methods. We found that brainstem hematoma gradually formed in the injured rats over the first 3 days and then reduced after 7 days. The edema that occurred was mainly of the vasogenic type. No complete myelin sheath structure was found around the focus of the brainstem hemorrhage. The integrity and continuity of nerve fibers gradually deteriorated over the first 7 days. Neuronal degeneration was mild in the first 3 days and then obviously aggravated on the 7th day. Inflammatory cytokines, interleukin-1β, and tumor necrosis factor α appeared on the 1st day after intracerebral hemorrhage, reached peak levels on the 3rd day, and decreased from the 7th day. Our findings show the characteristics of the progression of early brainstem hemorrhage.

Key words: brainstem hemorrhage, diffuse tensor imaging, diffusion-weighted imaging, Fluoro-Jade C staining, hematoxylin-eosin staining, interleukin-1β, luxol fast blue, rat model, T2-weighted imaging, tumor necrosis factor-α