中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1069-1075.doi: 10.4103/1673-5374.160095

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针预处理激活AMPK信号通路调动多种因素参与脑缺血损伤的保护

  

  • 收稿日期:2015-06-15 出版日期:2015-07-24 发布日期:2015-07-24

Electroacupuncture preconditioning attenuates ischemic brain injury by activation of the adenosine monophosphate-activated protein kinase signaling pathway

Qiang-qiang Ran, Huai-long Chen, Yan-li Liu, Hai-xia Yu, Fei Shi, Ming-shan Wang   

  1. Department of Anesthesiology, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao, Shandong Province, China
  • Received:2015-06-15 Online:2015-07-24 Published:2015-07-24
  • Contact: Ming-shan Wang, Ph.D., slwangmingshan@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81273821.

摘要:

电针对脑缺血损伤有治疗作用,但其作用机制尚不清楚,实验希望能解释这一现象。为此设计连续5d以1 mA,2/15 Hz,持续30 min电针刺激小鼠百会穴后,采用双侧颈总动脉结扎15min建立脑缺血模型。观察损伤后72 h经过电针预处理的小鼠海马神经元损伤明显减轻,TUNEL阳性细胞数量减少,且AMPKα和磷酸化AMPKα的表达上调;而腹腔注射AMPK拮抗剂化合物C可抑制上述现象。说明电针预处理激活了AMPK信号通路而发挥减轻脑缺血损伤的作用。

关键词: 神经再生, 电针, 脑缺血, 脑保护, AMPK, 化合物C, 神经元, 细胞凋亡

Abstract:

Electroacupuncture has therapeutic effects on ischemic brain injury, but its mechanism is still poorly understood. In this study, mice were stimulated by electroacupuncture at the Baihui (GV20) acupoint for 30 minutes at 1 mA and 2/15 Hz for 5 consecutive days. A cerebral ischemia model was established by ligating the bilateral common carotid artery for 15 minutes. At 72 hours after injury, neuronal injury in the mouse hippocampus had lessened, and the number of terminal deoxynucleotide transferase-mediated dUTP nick-end labeling-positive cells reduced after electroacupuncture treatment. Moreover, expression of adenosine monophosphate-activated protein kinase α (AMPKα) and phosphorylated AMPKα was up-regulated. Intraperitoneal injection of the AMPK antagonist, compound C, suppressed this phenomenon. Our findings suggest that electroacupuncture preconditioning alleviates ischemic brain injury via AMPK activation.

Key words: nerve regeneration, electroacupuncture, cerebral ischemia, neuroprotection, adenosine monophosphate-activated protein kinase α, compound C, neurons, apoptosis, NSFC grant, neural regeneration