中国神经再生研究(英文版) ›› 2016, Vol. 11 ›› Issue (6): 957-962.doi: 10.4103/1673-5374.184495

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

电针水沟穴治疗脑缺血再灌注损伤的另一种可能机制?

  

  • 收稿日期:2016-05-11 出版日期:2016-06-30 发布日期:2016-06-30
  • 基金资助:

    中国国家自然科学基金项目(81173339,81303020),长江学者和创新团队发展计划,天津市自然科学重点项目(11JCZDJC19800)

Large-conductance Ca2+-activated K+ channel involvement in suppression of cerebral ischemia/reperfusion injury after electroacupuncture at Shuigou (GV26) acupoint in rats

Yong Wang1, #, Yan Shen2, 5, 6, 7, #, Hai-ping Lin3, Zhuo Li4, Ying-ying Chen1, Shu Wang2, 5, 6, 7, *   

  1. 1 Postgraduate School of Tianjin University of Traditional Chinese Medicine, Tianjin, China 2 Institute of Acupuncture and Moxibustion Research, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 3 Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 4 Department of Rehabilitation Medicine, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China 5 Key Laboratory of Cerebropathy Acupuncture Therapy of State Administration of Traditional Chinese Medicine, Tianjin, China 6 Third-level Laboratory of Acupuncture Dose-Effect Relationship, State Administration of Traditional Chinese Medicine, Tianjin, China 7 Tianjin Key Laboratory of Acupuncture & Moxibustion Science, Tianjin, China
  • Received:2016-05-11 Online:2016-06-30 Published:2016-06-30
  • Contact: Shu Wang, wangs2008@163.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 81173339, 81303020; the Program for Changjiang Scholars and Innovative Research Team in University of China; the Key Project of the Natural Science Foundation of Tianjin of China, No.11JCZDJC19800.

摘要:

在脑缺血再灌注损伤中大电导钙激活钾离子通道过度激活与表达可能是神经元迟发性死亡的重要机制之一,电针治疗可以调控对于脑缺血再灌注损伤时大电导钙激活钾离子通道,但机制仍不明确。为此,实验建立脑缺血再灌注大鼠模型,以1mA、2Hz电针水沟穴(10min/次,每12h一次,72h内共6次)。发现电针刺激后脑缺血再灌注大鼠大脑皮质缺血性病理改变明显减轻,大脑皮质BKCa通道蛋白及mRNA表达减少,神经功能明显改善;且刺激水沟穴左侧旁开5mm处非穴位置时并不能出现上述变化。提示电针水沟穴改善脑缺血再灌注大鼠的神经功能评分,可能与下调大电导钙激活钾离子通道蛋白和mRNA的表达有关,同时提示该穴位存在一定的特异性。 

orcid: 0000-0002-3598-6055 (Shu Wang)

关键词: 神经再生, 电针, 水沟穴, 卒中, 大脑中动脉缺血再灌注, 大电导钙激活钾离子通道, 通道蛋白

Abstract:

Excess activation and expression of large-conductance Ca2+-activated K+ channels (BKCa channels) may be an important mechanism for delayed neuronal death after cerebral ischemia/reperfusion injury. Electroacupuncture can regulate BKCa channels after cerebral ischemia/ reperfusion injury, but the precise mechanism remains unclear. In this study, we established a rat model of cerebral ischemia/reperfusion injury. Model rats received electroacupuncture of 1 mA and 2 Hz at Shuigou (GV26) for 10 minutes, once every 12 hours for a total of six times in 72 hours. We found that in cerebral ischemia/reperfusion injury rats, ischemic changes in the cerebral cortex were mitigated after electroacupuncture. Moreover, BKCa channel protein and mRNA expression were reduced in the cerebral cortex and neurological function noticeably improved. These changes did not occur after electroacupuncture at a non-acupoint (5 mm lateral to the left side of Shuigou). Thus, our findings indicate that electroacupuncture at Shuigou improves neurological function in rats following cerebral ischemia/reperfusion injury, and may be associated with down-regulation of BKCa channel protein and mRNA expression. Additionally, our results suggest that the Shuigou acupoint has functional specificity.

Key words: nerve regeneration, electroacupuncture, Shuigou (GV26) acupoint, stroke, cerebral ischemia/reperfusion injury, large-conductance Ca2+-activated K+ channels, channel protein, neural regeneration