中国神经再生研究(英文版) ›› 2023, Vol. 18 ›› Issue (8): 1789-1794.doi: 10.4103/1673-5374.360247

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

脾切除不会影响小鼠行为表现

  

  • 出版日期:2023-08-15 发布日期:2023-02-24
  • 基金资助:
    国家自然科学基金项目(81772438,81974357),广州市科学技术计划项目(202206010197)

Splenectomy does not affect mouse behaviors

Jiao-Qiong Guan1, 2, #, Pei-Sen Zhang1, #, Wen-Chao Zhang3, Bing-Qian Zhang2, Hai-Tao Wu4, 5, *, Yue Lan1, 6, *, Ti-Fei Yuan2, 5, *   

  1. 1Department of Rehabilitation Medicine, Guangzhou First People’s Hospital, School of Medicine, South China University of Technology, Guangzhou, Guangdong Province, China; 2Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; 3College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China; 4Department of Neurobiology, Beijing Institute of Basic Medical Sciences, Beijing, China; 5Key Laboratory of Neuroregeneration, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu Province, China; 6Guangzhou Key Laboratory of Aging Frailty & Neurorehabilitation, Guangzhou, Guangdong Province, China
  • Online:2023-08-15 Published:2023-02-24
  • Contact: Ti-Fei Yuan, PhD, ytf0707@126.com; Yue Lan, PhD, bluemooning@163.com; Hai-Tao Wu, PhD, wuht@bmi.ac.cn.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81772438, 81974357, and the Guangzhou Municipal Science and Technology Program, No. 202206010197 (all to YL).

摘要:

脾脏对免疫至关重要,是机体内最大的免疫器官和免疫中心。既往对生理和疾病的研究已证实行为与免疫存在联系,但是脾脏是否也会影响行为,目前尚不清楚。为此,实验建立了脾切除小鼠模型,经旷场测试、昼夜节律测试、高架十字迷宫测试、蔗糖偏好试验、Barnes迷宫测试检测,可见脾切除对这些运动、昼夜节律、学习和记忆以及抑郁/焦虑相关行为没有显著影响。为进一步了解脾脏对应激敏感性的影响,以慢性不可预测轻度应激诱导小鼠建立抑郁模型,可见无脾小鼠模型与有脾小鼠模型的行为表现是相似的。这表明脾切除不会导致小鼠行为表现发生显著变化。

https://orcid.org/0000-0003-0510-715X (Ti-Fei Yuan); https://orcid.org/0000-0003-3367-7002 (Yue Lan); https://orcid.org/0000-0001-8437-3194 (Hai-Tao Wu)

关键词: 脾, 脾切除, 免疫, 慢性不可预测轻度应激, 昼夜节律, 运动, 抑郁, 焦虑, 学习, 记忆

Abstract: The spleen is critical for immunity. It is the largest immune organ and immune center in the peripheral system. While the relationship between behavior and immunity has been demonstrated in physiology and diseases, the role of the spleen in behavior is not clear. To investigate the effects of the spleen on behaviors, we performed a refined splenectomy procedure on C57BL/6J mice and performed an open field test, circadian rhythm test, elevated plus maze, sucrose preference test, and Barnes maze test. Splenectomy did not induce changes in general locomotion, circadian rhythms, learning and memory, or depression/anxiety-related behaviors. To further investigate the effects of spleen on stress susceptibility, we established mouse models of depression through chronic unpredictable mild stress. The behavioral performances of mice subjected to splenectomy showed no differences from control animals. These findings suggest that splenectomy does not cause changes in baseline behavioral performance in mice. 

Key words: anxiety, chronic unpredictable mild stress, circadian rhythms, depression, immunity, learning, locomotion, memory, spleen, splenectomy