中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2019, Vol. 14 ›› Issue (1): 107-113.doi: 10.4103/1673-5374.243714

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

针刺对急性期脑出血脑组织病理学、超微结构和神经功能影响的的时间效应

  

  • 出版日期:2019-01-15 发布日期:2019-01-15
  • 基金资助:

    天津市科学技术委员会（05YFSZSF02600；基金负责人：李平）

Time-effect relationship of acupuncture on histopathology, ultrastructure, and neuroethology in the acute phase of cerebral hemorrhage

Zuo-Wei Li 1, Xiao-Nan Zheng 2, Ping Li 3   

  1. 1 Shandong University of Traditional Chinese Medicine, Jinan, Shandong Province, China
    2 Department of Acupuncture, Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine, Tianjin, China
    3 Tianjin Third Central Hospital, Tianjin, China
  • Online:2019-01-15 Published:2019-01-15
  • Contact: Zuo-Wei Li, MD, 13820376327@163.com; Xiao-Nan Zheng, MD, snkgldnz@126.com.
  • Supported by:

    This study was supported by a grant from the Tianjin Science and Technology Commission, China, No. 05YFSZSF02600 (to PL).

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摘要：

针刺治疗急性期脑出血的临床研究很多，但是有关针刺时机与脑出血疗效之间的关系却少见报道。实验设计拟通过观察针刺对脑出血模型大鼠脑组织病理形态学、超微结构及神经行为学改变的影响，揭示针刺作用的时效性机制。(1)采用右侧尾状核自体血缓慢注入法制作脑出血模型大鼠，分为建模后3，9，24，48 h组，造模后3，9，24，48 h针刺组，针刺组选取双侧内关穴(PC6)、人中穴(DU26)每日针刺2次，每日间隔6 h，连续针刺5 d，同时设假手术组进行对比；(2)通过光学显微镜、透射电镜观察脑组织变化和Bederson和Longa评分进行神经行为学评估；(3)结果显示，与假手术组和模型组各组相比，针刺组各组Bederson和Longa评分降低，脑组织病理组织形态及超微结构均明显改善。3，9 h针刺组上述结果指标均优于模型组，各针刺组之间3，9 h针刺组上述结果指标均优于24，48 h针刺组；(4)上述数据说明，针刺治疗能够改善脑出血急性期所导致的相关病理、超微结构的恶化及神经功能缺损，在脑出血急性期起到保护脑组织的作用，且脑出血发生后越早(3或9 h)进行针刺干预疗效越好。

orcid: 0000-0001-888T-6311(Zuo-Wei Li)
           0000-0003-4039-0851(Xiao-Nan Zheng)

关键词: 针刺, 急性期, 脑出血, 时间效应, 超微结构, 功能, 组织病理学, 神经行为学, 脑损伤, 神经再生

Abstract:

Many clinical studies have addressed the treatment of acute cerebral hemorrhage using acupuncture. However, few studies have exam¬ined the relationship between time of acupuncture and curative effect on cerebral hemorrhage. By observing the effect of acupuncture on changes in histopathology, ultrastructure, and neuroethology in a cerebral hemorrhage model of rats, we have directly examined the time-effect relationship of acupuncture. The rat model of cerebral hemorrhage was produced by slowly injecting autologous blood to the right caudate nucleus. The experimental groups were: 3-, 9-, 24-, and 48-hour model groups; and 3-, 9-, 24-, and 48-hour acupuncture groups. The sham-operation group was used for comparison. Acupuncture was performed at the Neiguan (PC6) and Renzhong (DU26) acupoints, twice a day, 6 hours apart, for 5 consecutive days. Brain tissue changes were observed by light microscopy and transmission electron microscopy. Neuroethology was assessed using Bederson and Longa scores. Our results show that compared with the sham-op¬eration and model groups, Bederson and Longa scores were lower in each acupuncture group, with visibly improved histopathology and brain tissue ultrastructure. Further, the results were better in the 3- and 9-hour acupuncture groups than the 24- and 48-hour acupuncture groups. Our findings show that acupuncture treatment can relieve pathological and ultrastructural deterioration and neurological impair¬ment caused by the acute phase of cerebral hemorrhage, and may protect brain tissue during this period. In addition, earlier acupuncture intervention following cerebral hemorrhage (by 3 or 9 hours) is associated with a better treatment outcome.

Key words: nerve regeneration, acupuncture, acute phase, cerebral hemorrhage, time-effect, ultrastructure, function, histopathology, neuroethology, brain injury, neural regeneration