中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (2): 382-387.doi: 10.4103/1673-5374.290910

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

脊髓损伤小鼠系统性免疫反应的动态变化

  

  • 出版日期:2021-02-15 发布日期:2020-12-04
  • 基金资助:

    国家自然科学基金项目(81571213818005838160153981601084);国家重点研究发展计划项目(2017YFA0104304);国南京医学科技发展基金项目(QRX17006QRX17057);南京市卫健委和南京医科大学医学科技发展基金重点项目(201803024)和创新平台(ZDX16005

Dynamic changes in the systemic immune responses of spinal cord injury model mice

Tian-Yun Gao1, #, Fei-Fei Huang1, #, Yuan-Yuan Xie1, Wen-Qing Wang1, Liu-Di Wang1, Dan Mu2, Yi Cui3, Bin Wang1, *   

  1. 1 Clinical Stem Cell Center, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China; 2 Department of Radiology, the Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu Province, China; 3 Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China
  • Online:2021-02-15 Published:2020-12-04
  • Contact: Bin Wang, MD, wangbin022800@126.com. #Both authors contributed equally to this work.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 81571213 (to BW), 81800583 (to YYX), 81601539 (to DM), and 81601084 (to YC); the National Key Research and Development Program of China, No. 2017YFA0104304 (to BW); the Nanjing Medical Science and Technique Development Foundation of China, Nos. QRX17006 (to BW), QRX17057 (to DM); the Key Project Supported by Medical Science and Technology Development Foundation, Nanjing Department of Health and the Nanjing Medical Science of China, No. 201803024 (to TYG) and Innovation Platform, No. ZDX16005 (to BW).

摘要:

脊髓内炎症反应和免疫反应被认为在脊髓损伤的病理发展中起着关键作用。实验试图破译由脊髓损伤引发的系统免疫反应的动态变化。(1)实验完全横断小鼠T8脊髓,观察从急性期到亚急性期体内系统的炎症反应变化情况;(2)在急性期C反应蛋白、循环白细胞、淋巴细胞、脾源性CD4+ IFN-γ+T辅助细胞以及炎性细胞因子迅速下降,而中性粒细胞和单核细胞以及CD4+CD25+FOXP3+调节性T细胞明显增加,且这些系统性免疫反应在亚急性期逐渐恢复至基线水平;(3)表明在脊髓损伤急性期系统性免疫细胞和因子总体上呈现明显的抑制,而这种抑制是短暂的,这些严重失调的指标在亚急性期逐渐恢复到接近基线水平。实验于2019年6月25日经鼓楼医院实验动物中心伦理委员会批准,批准号2019AE01040。

https://orcid.org/0000-0003-3981-8849 (Bin Wang)

关键词:

脊髓损伤, 系统性免疫反应, 炎症, 免疫功能障碍, C反应蛋白, T辅助细胞, 调节性T细胞, 炎性细胞因子

Abstract: Intraspinal inflammatory and immune responses are considered to play central roles in the pathological development of spinal cord injury. This study aimed to decipher the dynamics of systemic immune responses, initiated by spinal cord injury. The spinal cord in mice was completely transected at T8. Changes in the in vivo inflammatory response, between the acute and subacute stages, were observed. A rapid decrease in C-reactive protein levels, circulating leukocytes and lymphocytes, spleen-derived CD4+ interferon-γ+ T-helper cells, and inflammatory cytokines, and a marked increase in neutrophils, monocytes, and CD4+CD25+FOXP3+ regulatory T-cells were observed during the acute phase. These systemic immune alterations were gradually restored to basal levels during the sub-acute phase. During the acute phase of spinal cord injury, systemic immune cells and factors showed significant inhibition; however, this inhibition was transient, and the indicators of these serious disorders gradually returned to baseline levels during the subacute phase. All experiments were performed in accordance with the institutional animal care guidelines, approved by the Institutional Animal Care and Use Committee of Experimental Animal Center of Drum Tower Hospital, China (approval No. 2019AE01040) on June 25, 2019.

Key words: C-reactive protein, immune dysfunction, inflammation, inflammatory cytokines, regulatory T-cells, spinal cord injury, systemic immune response, T-helper cells