中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2022, Vol. 17 ›› Issue (5): 1146-1155.doi: 10.4103/1673-5374.324860

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

加载长效控释神经生长因子的仿生壳聚糖支架修复成年大鼠长距离坐骨神经缺损

  

  • 出版日期:2022-05-15 发布日期:2021-11-22

Biomimetic chitosan scaffolds with long-term controlled release of nerve growth factor repairs 20-mm-long sciatic nerve defects in rats

Fa-Dong Liu1, Hong-Mei Duan2, Fei Hao1, Wen Zhao2, Yu-Dan Gao2, Peng Hao2, *, Zhao-Yang Yang2, 3, *, Xiao-Guang Li1, 2, 3, *   

  1. 1Beijing Key Laboratory for Biomaterials and Neural Regeneration, School of Biological Science and Medical Engineering, Beihang University, Beijing, China; 2Department of Neurobiology, Capital Medical University, Beijing, China; 3Beijing International Cooperation Bases for Science and Technology on Biomaterials and Neural Regeneration, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing, China
  • Online:2022-05-15 Published:2021-11-22
  • Contact: Peng Hao, PhD, hp_sarah@126.com; Zhao-Yang Yang, PhD, wack_lily@163.com; Xiao-Guang Li, PhD, lxgchina@sina.com.
  • Supported by:
    This study was supported by the National Natural Science Foundation of China, Nos. 31900749 (to PH), 31730030 (to XGL), 81941011 (to XGL), 31971279 (to ZYY), 31771053 (to HMD); and the Natural Science Foundation of Beijing of China, No. 7214301 (to FH).

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摘要：

自体神经移植是临床上治疗长距离周围神经损伤的金标准，但是这种治疗方法仍存在供体不足和二次损伤等缺点。加载控释神经生长因子的复合型壳聚糖支架可促进短距离坐骨神经损伤后神经元的存活和轴突再生，但对长距离神经缺损的治疗效果尚不明朗。实验在壳聚糖支架内加载了控释8周的神经生长因子，用于修复成年大鼠长距离（20 mm）坐骨神经缺损。结果显示，该支架可明显促进坐骨神经长距离缺损大鼠运动和感觉功能的恢复，且再生的坐骨神经不仅能与神经元重新建立连接，还可与中枢神经系统重建神经回路；另外，再生的坐骨神经还与靶肌肉重建了运动终板。因此这种新型仿生支架能够促进成年大鼠长距离坐骨神经缺损的再生和和功能回路重建，这将为长距离周围神经损伤的临床治疗提供一种有前景的方法。实验于2017年3月21日经首都医科大学动物伦理委员会批准（批准号AEEI-2017-033）。

https://orcid.org/0000-0002-2968-1052 (Fa-Dong Liu); https://orcid.org/0000-0002-8509-8232 (Peng Hao)

关键词: 周围神经损伤, 坐骨神经, 再生, 壳聚糖, 支架, 神经生长因子, 轴突, 髓鞘, 伪狂犬病毒, 功能恢复

Abstract: Although autogenous nerve transplantation is the gold standard for treating peripheral nerve defects of considerable length, it still has some shortcomings, such as insufficient donors and secondary injury. Composite chitosan scaffolds loaded with controlled release of nerve growth factor can promote neuronal survival and axonal regeneration after short-segment sciatic nerve defects. However, the effects on extended nerve defects remain poorly understood. In this study, we used chitosan scaffolds loaded with nerve growth factor for 8 weeks to repair long-segment (20 mm) sciatic nerve defects in adult rats. The results showed that treatment markedly promoted the recovery of motor and sensory functions. The regenerated sciatic nerve not only reconnected with neurons but neural circuits with the central nervous system were also reconstructed. In addition, the regenerated sciatic nerve reconnected the motor endplate with the target muscle. Therefore, this novel biomimetic scaffold can promote the regeneration of extended sciatic nerve defects and reconstruct functional circuits. This provides a promising method for the clinical treatment of extended peripheral nerve injury. This study was approved by the Animal Ethics Committee of Capital Medical University, China (approval No. AEEI-2017-033) on March 21, 2017. 

Key words: axon, chitosan, functional recovery, myelin sheath, nerve growth factor, peripheral nerve injury, pseudorabies virus, regeneration, scaffold, sciatic nerve

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