中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2021, Vol. 16 ›› Issue (9): 1829-1835.doi: 10.4103/1673-5374.306095

• 原著:脊髓损伤修复保护与再生 • 上一篇    下一篇

电活性材料氧化石墨烯复合壳聚糖支架有助于大鼠损伤脊髓的功能恢复

  

  • 出版日期:2021-09-15 发布日期:2021-02-05
  • 基金资助:
    国家重点研究发展计划项目（2018YFC1106800），四川省科技计划项目（2018JY0535），陆军医科大学人才培养计划项目（2019MPRC021/SWH2018QNWQ-05），光电磁声强度脑关键技术研究项目（AWS16J025）

Graphene oxide-composited chitosan scaffold contributes to functional recovery of injured spinal cord in rats

Bing Yang1, #, Pang-Bo Wang2, #, Ning Mu2, Kang Ma2, Shi Wang2, Chuan-Yan Yang2, Zhong-Bing Huang1, *, Ying Lai2, Hua Feng2, Guang-Fu Yin1, Tu-Nan Chen2, *, Chen-Shi Hu1   

  1. 1College of Biomedical Engineering, Sichuan University, Chengdu, Sichuan Province, China; 2Department of Neurosurgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing, China
  • Online:2021-09-15 Published:2021-02-05
  • Contact: Zhong-Bing Huang, PhD, zbhuang@scu.edu.cn; Tu-Nan Chen, PhD, ctn@tmmu.edu.cn.
  • Supported by:

    This work was supported by the National Key Research and Development Program of China, No. 2018YFC1106800 (to ZBH and GFY), Sichuan Science and Technology Project of China, No. 2018JY0535 (to ZBH), Talents Training Program of Army Medical University of China, No. 2019MPRC021/SWH2018QNWQ-05 (to TNC) and Research on Key Technologies of Photoelectromagnetic Acoustic Intensity Brain of China, No. AWS16J025 (to HF).

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摘要：

作者团队发现氧化石墨烯纳米片可赋予材料长达4周的持续导电性，导电性神经支架可桥接坐骨神经损伤并引导神经元生长，但其能否用于脊髓神经损伤的修复仍需探讨。（1）实验首先通过京尼平（Genipin）交联和冻干制备了一种氧化石墨烯复合壳聚糖支架。发现制备的支架具有多孔结构，内径约18‒87μm，支架中氧化石墨烯分布均匀，支架的导电性达到2.83 mS/cm，同时支架可被过氧化物酶降解。（2）将这种氧化石墨烯复合壳聚糖支架桥接修复T9全横断脊髓损伤大鼠模型，结果显示，这种支架能诱导神经细胞长入壳聚糖分子链间的孔隙中，同时可诱导再生组织中毛细血管的生成，促进神经元迁移和神经组织再生进入支架，从而促进损伤神经组织的修复。同时行为学及电生理学表明，氧化石墨烯复合壳聚糖支架可显著恢复大鼠的神经功能。并且氧化石墨烯的修复效果优于单纯壳聚糖支架。（3）结果表明氧化石墨烯可通过促进支架的降解、神经细胞黏附和迁移到支架，对脊髓损伤后神经功能的恢复产生积极的作用。实验于2019年8月30日经第三军医大学附属第一医院动物伦理委员会批准，批准号AMUWEC20191327。

https://orcid.org/0000-0002-3834-471X (Zhong-Bing Huang); https://orcid.org/0000-0003-0460-5632 (Tu-Nan Chen)

关键词:

脊髓损伤, 壳聚糖, 石墨烯, 导电, 支架, 血管生成, 修复, 再生

Abstract: The study illustrates that graphene oxide nanosheets can endow materials with continuous electrical conductivity for up to 4 weeks. Conductive nerve scaffolds can bridge a sciatic nerve injury and guide the growth of neurons; however, whether the scaffolds can be used for the repair of spinal cord nerve injuries remains to be explored. In this study, a conductive graphene oxide composited chitosan scaffold was fabricated by genipin crosslinking and lyophilization. The prepared chitosan-graphene oxide scaffold presented a porous structure with an inner diameter of 18–87 μm, and a conductivity that reached 2.83 mS/cm because of good distribution of the graphene oxide nanosheets, which could be degraded by peroxidase. The chitosan-graphene oxide scaffold was transplanted into a T9 total resected rat spinal cord. The results show that the chitosan-graphene oxide scaffold induces nerve cells to grow into the pores between chitosan molecular chains, inducing angiogenesis in regenerated tissue, and promote neuron migration and neural tissue regeneration in the pores of the scaffold, thereby promoting the repair of damaged nerve tissue. The behavioral and electrophysiological results suggest that the chitosan-graphene oxide scaffold could significantly restore the neurological function of rats. Moreover, the functional recovery of rats treated with chitosan-graphene oxide scaffold was better than that treated with chitosan scaffold. The results show that graphene oxide could have a positive role in the recovery of neurological function after spinal cord injury by promoting the degradation of the scaffold, adhesion, and migration of nerve cells to the scaffold. This study was approved by the Ethics Committee of Animal Research at the First Affiliated Hospital of Third Military Medical University (Army Medical University) (approval No. AMUWEC20191327) on August 30, 2019. 

Key words: angiogenesis, chitosan, electrical conduction, graphene oxide, regeneration, repair, scaffold, spinal cord injury ,