中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (30): 2827-2837.doi: 10.3969/j.issn.1673-5374.2013.30.005

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

人牙周韧带干细胞修复面神经损伤

  

  • 收稿日期:2013-06-25 修回日期:2013-08-26 出版日期:2013-10-25 发布日期:2013-10-25
  • 基金资助:

    韩国健康、福利与家庭事务部医学保健技术R&D项目（No. A101578）

Human periodontal ligament stem cells repair mental nerve injury

Bohan Li1, Hun-Jong Jung2, Soung-Min Kim3, Myung-Jin Kim3, Jeong Won Jahng4, Jong-Ho Lee3, 4   

  1. 1 Department of Oral and Maxillofacial Surgery, School of Dentistry, Binzhou Medical College, Binzhou, Shandong Province, China
    2 Department of Occupation and Environment, Konkuk Postgraduate Medical School, Choong-Ju, Korea
    3 Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea
    4 Dental Research Institute, Seoul National University, Seoul, Korea
  • Received:2013-06-25 Revised:2013-08-26 Online:2013-10-25 Published:2013-10-25
  • Contact: Jong-Ho Lee, D.D.S., M.S.D., Ph.D., Professor, Department of Oral and Maxillofacial Surgery, Clinical Trial Center and Oral Cancer Center, School of Dentistry, Seoul National University 275-1, Yeongun-Dong, Chongro-Ku, Seoul, 110-768, Korea, leejongh@snu.ac.kr.
  • Supported by:

    This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea, No. A101578.

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摘要：

基于人牙周韧带干细胞可分化为许旺样细胞和及易获取的特性。我们假设人牙周韧带干细胞可替代自体许旺细胞促进损伤周围神经再生。为验证该假设，给予左侧面神经挤压伤大鼠损伤部位注射1 × 106人牙周韧带干细胞，并以注射1×106自体许旺细胞和磷酸盐缓冲溶液PBS为对照。实时RT-PCR检测显示，干细胞注射5d后面神经损伤大鼠左侧三叉神经节低亲和力神经生长因子受体mRNA表达明显上调。感觉测试和三叉神经逆行性轴突传输及组织形态学分析显示，注射2和4周后，感觉功能明显改善，三叉神经感觉神经元和有髓(鞘)的轴突数量明显增加，且两种细胞移植效果相近。以上数据提示，以人牙周韧带干细胞移植在修复面神经损伤具有潜在价值。

关键词: 神经再生, 周围神经损伤, 干细胞, 牙周韧带干细胞, 面神经, 许旺细胞, 细胞移植, 感觉神经, 神经营养因子, 基金资助文章

Abstract:

Human periodontal ligament stem cells are easily accessible and can differentiate into Schwann cells. We hypothesized that human periodontal ligament stem cells can be used as an alternative source for the autologous Schwann cells in promoting the regeneration of injured peripheral nerve. To validate this hypothesis, human periodontal ligament stem cells (1 × 106) were injected into the crush-injured left mental nerve in rats. Simultaneously, autologous Schwann cells (1 × 106) and PBS were also injected as controls. Real-time reverse transcriptase polymerase chain reaction showed that at 5 days after injection, mRNA expression of low affinity nerve growth factor receptor was significantaly increased in the left trigeminal ganglion of rats with mental nerve injury. Sensory tests, histomorphometric evaluation and retrograde labeling demonstrated that at 2 and 4 weeks after in-jection, sensory function was significantly improved, the numbers of retrograde labeled sensory neurons and myelinated axons were significantly increased, and human periodontal ligament stem cells and autologous Schwann cells exhibited similar therapeutic effects. These findings suggest that transplantation of human periodontal ligament stem cells show a potential value in repair of mental nerve injury.

Key words: neural regeneration, peripheral nerve injury, stem cells, periodontal ligament stem cells, mental nerve, Schwann cells, cell transplantation, sensory nerve, neurotrophic factor, neuroregeneration