中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (3): 243-251.doi: 10.4103/1673-5374.128215

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

功能性电刺激促进脑梗死大鼠脑神经前体细胞的增殖与再生

  

  • 收稿日期:2013-11-06 出版日期:2014-02-15 发布日期:2014-02-15
  • 基金资助:

    国家自然科学基金(30772304, 30973166, 81171863)

Functional electrical stimulation-facilitated proliferation and regeneration of neural precursor cells in the brains of rats with cerebral infarction

Yun Xiang1, 2, Huihua Liu1, Tiebin Yan1, Zhiqiang Zhuang1, Dongmei Jin1, Yuan Peng3   

  1. 1 Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong Province, China
    2 Department of Rehabilitation Medicine, Shenzhen Sixth People’s Hospital, Shenzhen, Guangdong Province, China
    3 Department of Rehabilitation Medicine, Guangzhou First People’s Hospital, Guangzhou, Guangdong Province, China
  • Received:2013-11-06 Online:2014-02-15 Published:2014-02-15
  • Contact: Tiebin Yan, Ph.D., Department of Rehabilitation Medicine, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China, tbyan@hotmail.com.
  • Supported by:

    The research was supported by the National Natural Science Foundation of China, grants No. 30772304, 30973166, and 81171863.

摘要:

目前的研究表明,内源性神经前体细胞增殖程度不足以代偿脑卒中时神经元和轴突的受损。实验从神经可塑性角度入手,观察功能性电刺激治疗对急性脑梗死大鼠脑部内源性神经前体细胞的增殖和梗死侧碱性成纤维细胞生长因子、表皮生长因子表达的影响。给予急性大脑中动脉梗死模型大鼠功能性电刺激治疗,并同时设安慰刺激组和假手术组作对照。免疫组织化学染色结果显示,治疗第7、14天,与安慰刺激组相比,电刺激治疗组大鼠海马齿状回和室管膜下区神经前体细胞标志物-巢蛋白的表达阳性细胞数增加。Western-blot和酶联免疫吸附检测显示,电刺激治疗组梗死侧脑组织碱性成纤维细胞生长因子、表皮生长因子总蛋白和基因表达量在治疗第7、14天高于安慰刺激组。网屏试验结果显示,治疗第14天,电刺激治疗组大鼠抓握功能明显好于安慰刺激组。结果证实,功能性电刺激能促进急性脑梗死大鼠脑部内源性神经前体细胞的增殖与再生,使碱性成纤维细胞生长因子及表皮生长因子的表达增加,并改善了大鼠的运动功能。

关键词: 神经再生, 脑损伤, 功能性电刺激, 神经前体细胞, 神经发生, 碱性成纤维细胞生长因子, 表皮生长因子, 巢蛋白, 脑卒中, 大鼠, 国家自然科学基金

Abstract:

Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plasticity, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.

Key words: nerve regeneration, brain injury, functional electrical stimulation, neural precursor cells, neurogenesis, basic fibroblast growth factor, epidermal growth factor, nestin, stroke, rats, NSFC grant, neural regeneration