中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (9): 978-985.doi: 10.4103/1673-5374.133151

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

液压冲击损伤大鼠海马神经元后3种因子时序性表达的规律与特点

  

  • 收稿日期:2014-03-10 出版日期:2014-05-20 发布日期:2014-05-20
  • 基金资助:

    国家自然科学基金项目(No.30471934)资助

Sequential expression of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor in rat hippocampal neurons after fluid percussion injury

Zhiqiang Li 1, Qingming Shu 2, Lingzhi Li 3, Maolin Ge 4, Yongliang Zhang 5   

  1. 1 Second Department of Medicine, Inner Mongolia Corps Hospital, Chinese People’s Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China
    2 Department of Pathology, General Hospital of Chinese People’s Armed Police Forces, Beijing, China
    3 Section of Pharmaceutical Chemistry, Department of Rescue Medicine, Logistics University of Chinese People’s Armed Police Force, Tianjin, China
    4 Second Department of Surgery, Inner Mongolia Corps Hospital, Chinese People’s Armed Police Forces, Huhhot, Inner Mongolia Autonomous Region, China
    5 Training Department, Logistics University of Chinese People’s Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin, China 
  • Received:2014-03-10 Online:2014-05-20 Published:2014-05-20
  • Contact: Yongliang Zhang, M.D., Training Department, Logistics University of Chinese People’s Armed Police Force, Tianjin Key Laboratory for Biomarkers of Occupational and Environmental Hazard, Tianjin 300300, China, zhang78127@tom.com.
  • Supported by:

    This study was supported by the National Natural Science Foundation of China, No. 30471934.

PDF

814

摘要：

创伤性脑损伤可引起不同脑区基因表达改变，其中环氧化酶2、谷氨酸受体2和血小板活化因子受体3种因子的表达与创伤性脑损伤的发生与发展相关，但与脑神经元损伤的具体关系尚不明确。实验以原代培养大鼠海马神经元为观察对象，建立改良Scott′s中度液压冲击神经元损伤模型。RT-PCR和免疫细胞化学染色半定量检测结果发现，液压冲击损伤大鼠海马神经元中的环氧化酶2 、谷氨酸受体2和血小板活化因子受体 mRNA及蛋白表达均呈先增高后降低的趋势，且于损伤后8，12 h表达最显著。血小板活化因子受体、环氧化酶-2和谷氨酸受体2在闭合性颅脑损伤海马组织中的表达时序性变化特点基本一致，在损伤后8，12 h这3个因子表达最显著，共同参与海马神经元损伤的发生与发展。

关键词: 神经再生, 脑损伤, 血小板活化因子, 环氧化酶-2, 反转录-聚合酶链反应, 免疫细胞化学染色, 大鼠, 海马, 脑损伤, 血小板活化因子受体, 谷氨酸受体2, 国家自然科学基金

Abstract:

Traumatic brain injury causes gene expression changes in different brain regions. Occurrence and development of traumatic brain injury are closely related, involving expression of three factors, namely cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. However, little is known about the correlation of these three factors and brain neuronal injury. In this study, primary cultured rat hippocampal neurons were subjected to fluid percussion injury according to Scott’s method, with some modifications. RT-PCR and semi-quantitative immunocytochemical staining was used to measure the expression levels of cyclooxygenase-2, glutamate receptor-2, and platelet activating factor receptor. Our results found that cyclooxygenase-2 expression were firstly increased post-injury, and then decreased. Both mRNA and protein expression levels reached peaks at 8 and 12 hours post-injury, respectively. Similar sequential changes in glutamate receptor 2 were observed, with highest levels mRNA and protein expression at 8 and 12 hours post-injury respectively. On the contrary, the expressions of platelet activating factor receptor were firstly decreased post-injury, and then increased. Both mRNA and protein expression levels reached the lowest levels at 8 and 12 hours post-injury, respectively. Totally, our findings suggest that these three factors are involved in occurrence and development of hippocampal neuronal injury.