中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (11): 965-974.doi: 10.3969/j.issn.1673-5374.2013.11.001

• 原著:周围神经损伤修复保护与再生 •    下一篇

硫酸软骨素酶ABC联合骨髓间充质干细胞修复脊髓损伤

  

  • 收稿日期:2012-09-29 修回日期:2013-01-20 出版日期:2013-04-15 发布日期:2013-04-15

Chondroitinase ABC plus bone marrow mesenchymal stem cells for repair of spinal cord injury

Chun Zhang, Xijing He, Haopeng Li, Guoyu Wang   

  1. Department of Orthopedics, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2012-09-29 Revised:2013-01-20 Online:2013-04-15 Published:2013-04-15
  • Contact: Xijing He, Ph.D., Professor, Doctoral supervisor, Department of Orthopedics, Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China, Xijing_h@tom.com.
  • About author:Chun Zhang☆, Ph.D., Associate professor

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摘要：

硫酸软骨素酶ABC可以改善脊髓损伤后局部不良微环境，细胞移植也被证明是一个不错的选择。假设两者联合干预更有利于脊髓损伤的治疗，实验于大鼠T8脊髓挤压伤后第5天，在脊髓损伤区边缘1mm分别注射骨髓间充质干细胞悬液或硫酸软骨素酶ABC。联合移植组先注射硫酸软骨素酶ABC，第2天注射骨髓间充质干细胞悬浮液。脊髓损伤后14天，联合移植组的平均BBB评分明显高于其他组。苏木精-伊红染色显示，联合移植组的损伤坏死面积与其他组相比明显较小。胶质纤维酸性蛋白-硫酸软骨素蛋白多糖双重免疫染色显示，联合移植组星形胶质细胞瘢痕损伤区明显缩小，而且没有空洞形成。胶质纤维酸性蛋白-生长相关蛋白43免疫组化染色显示，联合移植组阳性神经纤维通过损伤区。说明联合硫酸软骨素酶ABC和骨髓间充质干细胞移植更利于脊髓损伤修复。

关键词: 神经再生, 脊髓损伤, 干细胞, 硫酸软骨素蛋白多糖, 星形胶质细胞, 神经胶质瘢痕, 硫酸软骨素酶ABC, 骨髓间充质干细胞, 移植, 化学屏障

Abstract:

As chondroitinase ABC can improve the hostile microenvironment and cell transplantation is proven to be effective after spinal cord injury, we hypothesized that their combination would be a more effective treatment option. At 5 days after T8 spinal cord crush injury, rats were injected with bone marrow mesenchymal stem cell suspension or chondroitinase ABC 1 mm from the edge of spinal cord damage zone. Chondroitinase ABC was first injected, and bone marrow mesenchymal stem cell suspension was injected on the next day in the combination group. At 14 days, the mean Basso, Beattie and Bresnahan score of the rats in the combination group was higher than other groups. Hematoxylin-eosin staining showed that the necrotic area was significantly reduced in the combination group compared with other groups. Glial fibrillary acidic protein-chondroitin sulfate proteoglycan double staining showed that the damage zone of astrocytic scars was significantly reduced without the cavity in the combination group. Glial fibrillary acidic protein/growth associated protein-43 double immunostaining revealed that positive fibers traversed the damage zone in the combination group. These results suggest that the combination of chondroitinase ABC and bone marrow mesenchymal stem cell transplantation contributes to the repair of spinal cord injury.

Key words: neural regeneration, spinal cord injury, stem cells, chondroitin sulfate proteoglycans, astrocytes, glial scar, chondroitinase ABC, bone marrow mesenchymal stem cells, transplantation, chemical barrier, neuroregeneration