中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (12): 1081-1090.doi: 10.3969/j.issn.1673-5374.2013.12.003

• 原著:退行性病与再生 • 上一篇    下一篇

中药黄芩茎叶总黄酮能减轻淀粉样β肽诱导大鼠海马神经元的凋亡的证据

  

  • 收稿日期:2012-12-25 修回日期:2013-03-05 出版日期:2013-04-25 发布日期:2013-04-25

Scutellaria baicalensis stem-leaf total flavonoid reduces neuronal apoptosis induced by amyloid-beta peptide (25–35)

Ruiting Wang1, Xingbin Shen2, Enhong Xing2, Lihua Guan1, Lisheng Xin2   

  1. 1 Department of Pharmacology, Chengde Medical College, Chengde 067000, Hebei Province, China
    2 Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China
  • Received:2012-12-25 Revised:2013-03-05 Online:2013-04-25 Published:2013-04-25
  • Contact: Ruiting Wang☆, Ph.D., Professor, Master’s supervisor, Department of Pharmacology, Chengde Medical College, Chengde 067000, Hebei Province, China, Wrtwrt8324@ sina.com.

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摘要：

前期研究显示黄芩茎叶总黄酮能减轻淀粉样β肽引起的大鼠学习记忆能力损伤和海马神经元脱失。在此基础上，作者的工作又进一步观察了黄芩茎叶总黄酮对淀粉样β肽诱导的大鼠海马神经元凋亡及凋亡相关蛋白表达的影响。向Wistar大鼠灌胃50，100mg/kg黄芩茎叶总黄酮，1次/d，灌胃第8天向大鼠双侧海马各注射10μg淀粉样β肽25-35诱导神经元凋亡。灌胃20d后，取大鼠海马组织。TUNEL染色显示，50，100mg/kg黄芩茎叶总黄酮均可减少淀粉样β肽25-35诱导的大鼠海马细胞凋亡；免疫组化和western blot结果显示，50，100mg/kg黄芩茎叶总黄酮可抑制淀粉样β肽25-35诱导的大鼠海马组织促凋亡蛋白Bax及有级联反应关系的细胞色素c、Caspase-3的表达，促进抑凋亡蛋白Bcl-2的表达，以100mg/kg黄芩茎叶总黄酮的作用更明显。作者认为，黄芩茎叶总黄酮可剂量依赖性减轻淀粉样β肽诱导的海马细胞凋亡，其作用与调控凋亡相关蛋白Bcl-2、Bax、细胞色素c、Caspase-3的表达有关。

关键词: 神经再生, 中医药, 神经退行性疾病, 黄芩茎叶总黄酮, 淀粉样β肽, 神经元, 凋亡蛋白, 细胞色素c, 阿尔茨海默病, 基金资助文章

Abstract:

Scutellaria baicalensis stem-leaf total flavonoid might attenuate learning/memory impairment and neuronal loss in rats induced by amyloid beta-peptide. This study aimed to explore the effects of Scutellaria baicalensis stem-leaf total flavonoid on amyloid beta-peptide-induced neuronal apoptosis and the expression of apoptosis-related proteins in the rat hippocampus. Male Wistar rats were given intragastric administration of Scutellaria baicalensis stem-leaf total flavonoid, 50 or 100 mg/kg, once per day. On day 8 after administration, 10 μg amyloid beta-peptide (25–35) was injected into the bilateral hippocampus of rats to induce neuronal apoptosis. On day 20, hippocampal tissue was harvested and probed with the terminal deoxyribonucleotidyl transferase-mediated biotin-16-dUTP nick-end labeling assay. Scutellaria baicalensis stem-leaf total flavonoid at 50 and 100 mg/kg reduced neuronal apoptosis induced by amyloid beta-peptide (25–35) in the rat hippocampus. Immunohistochemistry and western blot assay revealed that expression of the pro-apoptotic protein Bax, cytochrome c and caspase-3 was significantly diminished by 50 and 100 mg/kg Scutellaria baicalensis stem-leaf total flavonoid, while expression of the anti-apoptotic protein Bcl-2 was increased. Moreover, 100 mg/kg Scutellaria baicalensis stem-leaf total flavonoid had a more dramatic effect than the lower dosage. These experimental findings indicate that Scutellaria baicalensis stem-leaf total flavonoid dose-dependently attenuates neuronal apoptosis induced by amyloid beta-peptide in the hippocampus, and it might mediate this by regulating the expression of Bax, cytochrome c, caspase-3 and Bcl-2.