中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (33): 3124-3130.doi: 10.3969/j.issn.1673-5374.2013.33.006

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

坐骨神经损伤大鼠背根神经节内P物质和降钙素基因相关肽的表达

  

  • 收稿日期:2013-04-30 修回日期:2013-06-13 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:

    教育部科学技术研究重点基金资助项目 （207049）

Substance P and calcitonin gene-related peptide expression in dorsal root ganglia in sciatic nerve injury rats

Changma Fu1, Zongsheng Yin1, Defu Yu1, Zuhua Yang2   

  1. 1 Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
    2 Third Clinical College, Anhui Medical University, Hefei 230022, Anhui Province, China
  • Received:2013-04-30 Revised:2013-06-13 Online:2013-11-25 Published:2013-11-25
  • Contact: Zongsheng Yin, M.D., Chief physician, Department of Orthopedics, First Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China, yinzongsheng@sina.com.
  • About author:Changma Fu, M.D., Attending physician.
  • Supported by:

    This study was supported by the Major Program of Science and Technology of Ministry of Education, No. 207049.

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摘要：

于雌性SD大鼠左侧股中部切断坐骨神经，并于断端套入硅胶管，制备坐骨神经缺损模型。分别于坐骨神经损伤后7,14,28d取大鼠L5背根神经节进行免疫组化染色，结果显示坐骨神经损伤大鼠背根神经节内P物质和降钙素基因相关肽的表达明显增高，于坐骨神经损伤后第7天达高峰，14d有所下降，28d时降至正常水平。可见神经肽P物质和降钙素基因相关肽主要在坐骨神经损伤早期表达增加。

关键词: 坐骨神经, 周围神经损伤, 背根神经节, 脊髓, 神经肽, 降钙素基因相关肽, P物质, 疼痛, 神经保护, 神经再生

Abstract:

The neuropeptides, substance P and calcitonin gene-related peptide, have been shown to be involved in pain transmission and repair of sciatic nerve injury. A model of sciatic nerve defect was prepared by dissecting the sciatic nerve at the middle, left femur in female Sprague Dawley rats. The two ends of the nerve were encased in a silica gel tube. L5 dorsal root ganglia were harvested 7, 14 and 28 days post sciatic nerve injury for immunohistochemical staining. Results showed that substance P and cal-citonin gene-related peptide expression increased significantly in dorsal root ganglion of rats with sci-atic nerve injury. This increase peaked at 7 days, declined at 14 days, and reduced to normal levels by 28 days post injury. The findings indicate that the neuropeptides, substance P and calcitonin gene- related peptide, mainly increased in the early stages after sciatic nerve injury.

Key words: neural regeneration, peripheral nerve injury, sciatic nerve, dorsal root ganglion, spinal cord, neuropeptides, calcitonin gene-related peptide, substance P, pain, neuroprotection, grants- supported paper, neuroregeneration