中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2013, Vol. 8 ›› Issue (33): 3139-3147.doi: 10.3969/j.issn.1673-5374.2013.33.008

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

壳聚糖导管复合神经生长因子微球可有效修复面神经缺损

  

  • 收稿日期:2013-04-01 修回日期:2013-06-10 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:

    国家自然科学基金项目（30872898, 81371116 ）北京自然科学基金项目（7132173）

Chitosan conduits combined with nerve growth factor microspheres repair facial nerve defects

Huawei Liu1, Weisheng Wen1, Min Hu1, Wenting Bi2, Lijie Chen3, Sanxia Liu1, Peng Chen1, Xinying Tan1   

  1. 1 Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China
    2 Department of Stomatology, Second Hospital of Beijing Chaoyang District, Beijing 100026, China
    3 Department of Stomatology, First Sanatorium of Qingdao, Jinan Military Area Command of Chinese PLA, Qingdao 266071, Shandong Province, China
  • Received:2013-04-01 Revised:2013-06-10 Online:2013-11-25 Published:2013-11-25
  • Contact: Weisheng Wen, Master, Associate professor, Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China, wenws_pla@sohu.com. Min Hu, M.D, Ph.D., Institute of Stomatology, Chinese PLA General Hospital, Beijing 100853, China, humin48@vip.163.com.
  • About author:Huawei Liu, M.D.

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摘要：

实验通过复合法制备神经生长因子缓释微球，并将其植入壳聚糖导管中以提供持续释放的神经生长因子，用其修复兔右侧面神经颊支10mm缺损，并以壳聚糖导管复合神经生长因子或生理盐水及自体神经修复作为对照。面神经修复90d，.见生理盐水组和神经生长因子组面部肌肉萎缩较神经生长因子微球组和自体神经移植组明显。神经电生理检测发现神经生长因子微球组和自体神经移植组的神经传导速度和振幅高于生理盐水组和神经生长因子组。同时组织学观察结果显示神经生长因子微球组和自体神经移植组神经纤维直径、数量、密度及髓鞘厚度均高于生理盐水组和神经生长因子组。说明壳聚糖导管复合神经生长因子缓释微球对兔面神经缺损的修复有明显的促进作用。

关键词: 神经再生, 组织工程, 神经生长因子, 微球, 面神经缺损, 壳聚糖, 神经导管, 周围神经, 脑神经, 神经损伤, 基金资助文章

Abstract:

Microspheres containing nerve growth factor for sustained release were prepared by a compound method, and implanted into chitosan conduits to repair 10-mm defects on the right buccal branches of the facial nerve in rabbits. In addition, chitosan conduits combined with nerve growth factor or normal saline, as well as autologous nerve, were used as controls. At 90 days post-surgery, the muscular atrophy on the right upper lip was more evident in the nerve growth factor and normal sa-line groups than in the nerve growth factor-microspheres and autologous nerve groups. Electro-physiological analysis revealed that the nerve conduction velocity and amplitude were significantly higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups. Moreover, histological observation illustrated that the di-ameter, number, alignment and myelin sheath thickness of myelinated nerves derived from rabbits were higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups. These findings indicate that chitosan nerve conduits com-bined with microspheres for sustained release of nerve growth factor can significantly improve facial nerve defect repair in rabbits.

Key words: neural regeneration, peripheral nerve injury, tissue engineering, nerve growth factor, microsphere, facial nerve defect, chitosan, nerve conduit, grants-supported paper, neuroregeneration