中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (2): 119-128.doi: 10.4103/1673-5374.125339

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

骨形态发生蛋白4诱导少突胶质前体细胞生成的II型星形胶质细胞对背根节神经元的影响

  

  • 收稿日期:2013-11-22 出版日期:2014-01-10 发布日期:2014-01-10
  • 基金资助:

    美国NIH基金(R01 NS061975);湖南省自然科学基金(11JJ6077)

Effect of type-2 astrocytes on the viability of dorsal root ganglion neurons and length of neuronal processes

Chunling Fan1, Hui Wang1, Dan Chen1, Xiaoxin Cheng2, Kun Xiong1, Xuegang Luo1, Qilin Cao2   

  1. 1 Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha, Hunan Province, China
    2 The Vivian L Smith Department of Neurosurgery, UT Medical School at Houston, Houston, TX, USA
  • Received:2013-11-22 Online:2014-01-10 Published:2014-01-10
  • Contact: Xuegang Luo, Department of Human Anatomy and Neurobiology, Xiangya School of Medicine, Central South University, Changsha 410013, Hunan Province, China, xgluo@xysm.net. Qilin Cao, The Vivian L Smith Department of Neurosurgery, UT Medical School at Houston, Houston, TX 77030, USA, qi-lin.cao@uth.tmc.edu.
  • Supported by:

    This study was supported by the NIH Foundation of the USA, No. R01 NS061975, and the Natural Science Foundation of Hunan Province in China, No. 11JJ6077.

摘要:

中枢神经系统病变发生后,病灶周围的骨形态发生蛋白的浓度明显升高,骨形态发生蛋白可以有效抑制少突胶质前体细胞向少突胶质细胞分化,诱导其分化为II型星形胶质细胞,II型星形胶质细胞在中枢神经系统损伤修复中扮演什么样的角色,目前尚不明确。实验在相对单纯的体外培养条件下,把骨形态发生蛋白4诱导少突胶质前体细胞分化形成的II型星形胶质细胞与脊髓背根节神经元共培养,观察脊髓背根节神经元的存活及突起的生长情况,比较分析少突胶质前体细胞及其分化的II型星形胶质细胞对脊髓背根节神经元生长的影响,探索影响少突胶质前体细胞细胞替代治疗中枢神经系统脱髓鞘病变疗效的可能因素。结果发现与少突胶质前体细胞和II型星形胶质细胞共培养的脊髓背根节神经元的存活细胞数比未进行细胞共培养的高,比与I型星形胶质细胞共培养的低;神经元最长突起长度和总突起长度比较发现,未进行细胞共培养的最短,与II型星形胶质细胞共培养的次之,与少突胶质前体细胞共培养的较高,与I型星形胶质细胞共培养的最高。说明细胞接触培养可增加神经元的存活率和突起长度,与I型星形胶质细胞和少突胶质前体细胞相比,II型星形胶质细胞抑制脊髓背根节神经元突起生长。

关键词: 神经再生, 脊髓损伤, 少突胶质细胞(Oligodendroglia), 少突胶质前体细胞, 星形胶质细胞(Astrocytes), 骨形态发生蛋白(Bone Morphogenetic Proteins), 神经元, 神经突(Neurites), 背根节, 美国NIH基金

Abstract:

The role of type-2 astrocytes in the repair of central nervous system injury remains poorly understood. In this study, using a relatively simple culture condition in vitro, type-2 astrocytes, differentiated from oligodendrocyte precursor cells by induction with bone morphogenetic protein-4, were co-cultured with dorsal root ganglion neurons. We examined the effects of type-2 astrocytes differentiated from oligodendrocyte precursor cells on the survival and growth of dorsal root ganglion neurons. Results demonstrated that the number of dorsal root ganglion neurons was higher following co-culture of oligodendrocyte precursor cells and type-2 astrocytes than when cultured alone, but lower than that of neurons co-cultured with type-1 astrocytes. The length of the longest process and the length of all processes of a single neuron were shortest in neurons cultured alone, followed by neurons co-cultured with type-2 astrocytes, then neurons co-cultured with oligodendrocyte precursor cells, and longest in neurons co-cultured with type-1 astrocytes. These results indicate that co-culture with type-2 astrocytes can increase neuronal survival rate and process length. However, compared with type-1 astrocytes and oligodendrocyte precursor cells, the promotion effects of type-2 astrocytes on the growth of dorsal root ganglion neurons were weaker.

Key words: nerve regeneration, spinal cord injury, oligodendrocyte, oligodendrocyte precursor cells, astrocytes, bone morphogenetic protein, neurons, neurites, dorsal root ganglion, NIH grant, neural regeneration