中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (10): 1041-1046.doi: 10.4103/1673-5374.133166

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

一个简单桡神经损伤猕猴模型评估周围神经损伤的修复

  

  • 收稿日期:2014-03-10 出版日期:2014-05-26 发布日期:2014-05-26
  • 基金资助:

    基金资助: 863项目(2006AA02A130), 国家自然科学基金(81372041, 31070869, 30700847)

A simple model of radial nerve injury in the rhesus monkey to evaluate peripheral nerve repair

Dong Wang, Xijun Huang, Guo Fu, Liqiang Gu, Xiaolin Liu, Honggang Wang, Jun Hu, Jianhua Yi, Xiaofeng Niu, Qingtang Zhu   

  1. Department of Orthopedics and Microsurgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong Province, China
  • Received:2014-03-10 Online:2014-05-26 Published:2014-05-26
  • Contact: Qingtang Zhu, M.D., Ph.D., Department of Orthopedics and Microsurgery, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510080, Guangdong Province, China, qtzhu@qq.com.
  • Supported by:

    This study was supported by the National High-Technology Research and Development Program of China (863 Program), No. 2006AA02A130; the National Natural Science Foundation of China, No. 81372041, 31070869, 30700847.

摘要:

关于骨髓基质干细胞移植和自体或异体神经移植修复周围神经损伤的动物模型研究目前主要局限于啮齿类动物。实验建立了一种更接近人的标准灵长类动物周围神经缺损模型,并可进一步研究周围神经损伤的修复效果。实验以自体神经移植物、同种异体神经与自体骨髓基质干细胞复合体及单独同种异体神经3种方法修复2.5 cm长猕猴桡神经缺损。移植后5个月,伸腕实验、组织学和神经电生理检测显示,与单纯同种异体神经移植比较,骨髓基质干细胞和同种异体神经联合移植后猕猴桡神经功能恢复更明显,可促进移植后的桡神经远端轴突的生长,神经元存活百分比,神经纤维密度及直径、髓鞘厚度、神经传导速度以及复合动作电位峰值均增高,且修复效果与自体神经移植相当。结果证实,骨髓基质干细胞和同种异体神经复合物联合移植修复猕猴桡神经损伤,能达到和自体神经移植相似的修复效果,同时实验成功建立了一种简单的猕猴桡神经缺损模型,可用来评估桡神经损伤修复的效果。

关键词: 神经再生, 周围神经损伤, 猕猴, 骨髓基质干细胞, 同种异体神经, 移植, 腕伸试验, 神经电生理, 神经功能, 国家自然科学基金

Abstract:

Current research on bone marrow stem cell transplantation and autologous or xenogenic nerve transplantation for peripheral nerve regeneration has mainly focused on the repair of peripheral nerve defects in rodents. In this study, we established a standardized experimental model of radial nerve defects in primates and evaluated the effect of repair on peripheral nerve injury. We repaired 2.5-cm lesions in the radial nerve of rhesus monkeys by transplantation of autografts, acellular allografts, or acellular allografts seeded with autologous bone marrow stem cells. Five months after surgery, regenerated nerve tissue was assessed for function, electrophysiology, and histomorphometry. Postoperative functional recovery was evaluated by the wrist-extension test. Compared with the simple autografts, the acellular allografts and allografts seeded with bone marrow stem cells facilitated remarkable recovery of the wrist-extension functions in the rhesus monkeys. This functional improvement was coupled with radial nerve distal axon growth, a higher percentage of neuron survival, increased nerve fiber density and diameter, increased myelin sheath thickness, and increased nerve conduction velocities and peak amplitudes of compound motor action potentials. Furthermore, the quality of nerve regeneration in the bone marrow stem cells-laden allografts group was comparable to that achieved with autografts. The wrist-extension test is a simple behavioral method for objective quantification of peripheral nerve regeneration.

Key words: nerve regeneration, peripheral nerve injury, rhesus monkeys, bone marrow stem cells, allogeneic nerve, transplantation, wrist-extension test, electrophysiology, neurological function, NSFC grant, neural regeneration