中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (11): 1117-1121.doi: 10.4103/1673-5374.135313

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

缺血预处理怎样保护脑梗死:与血管内皮生长因子上调有关?

  

  • 收稿日期:2014-04-01 出版日期:2014-06-12 发布日期:2014-06-12

Neuroprotective effect of ischemic preconditioning in focal cerebral infarction: relationship with upregulation of vascular endothelial growth factor

Yong Liu 1, 2, Suiqiang Zhu 1, Yunfu Wang 2, Jingquan Hu 2, Lili Xu 2, Li Ding 2, Guangjian Liu 2   

  1. 1 Department of Neurology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China
    2 Department of Neurology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan, Hubei Province, Chi-na
  • Received:2014-04-01 Online:2014-06-12 Published:2014-06-12
  • Contact: Yong Liu, Ph.D., Department of Neurology, Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, Hubei Province, China, taihly@sina.com

Abstract:

Neuroprotection by ischemic preconditioning has been confirmed by many studies, but the precise mechanism remains unclear. In the present study, we performed cerebral ischemic preconditioning in rats by simulating a transient ischemic attack twice (each a 20-minute occlusion of the middle cerebral artery) before inducing focal cerebral infarction (2 hour occlusion-reperfusion in the same artery). We also explored the mechanism underlying the neuroprotective effect of ischemic preconditioning. Seven days after occlusion-reperfusion, tetrazolium chloride staining and immunohistochemistry revealed that the infarct volume was significantly smaller in the group that underwent preconditioning than in the model group. Furthermore, vascular endothelial growth factor immunoreactivity was considerably greater in the hippocampal CA3 region of preconditioned rats than model rats. Our results suggest that the protective effects of ischemic preconditioning on focal cerebral infarction are associated with upregulation of vascular endothelial growth factor.

Key words: nerve regeneration, brain injury, transient ischemic attack, ischemic preconditioning, ischemia-reperfusion, focal cerebral infarction, infarct volume ratio, vascular endothelial growth , factor, protection, mechanism, neural regeneration 

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