中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (13): 1303-1312.doi: 10.4103/1673-5374.137578

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

青春期大鼠视前区促性腺激素释放激素神经元的形态变化

  

  • 收稿日期:2014-05-12 出版日期:2014-07-11 发布日期:2014-07-11

Morphological changes of gonadotropin-releasing hormone neurons in the rat preoptic area across puberty

Haogang Xue 1, Xiaodong Gai 2, Weiqi Sun 3, Chun Li 2, Quan Liu 4   

  1. 1 Department of Orthopedic Surgery, Affiliated Hospital of Beihua University, Changchun, Jilin Province, China
    2 Department of Pathology, Beihua University, Changchun, Jilin Province, China
    3 College of Public Health, Beihua University, Changchun, Jilin Province, China
    4 Department of Cardiovascular Disease, the First Hospital of Jilin University, Changchun, Jilin Province, China
  • Received:2014-05-12 Online:2014-07-11 Published:2014-07-11
  • Contact: Quan Liu, Ph.D., Department of Cardiovascular Disease, the First Hospital of Jilin University, Changchun, Jilin Province, China, quanliu888@163.com.

摘要:

视前区促性腺激素释放激素神经元在青春期时表现活跃,其形态也可能发生变化。实验以共聚焦激光扫描显微镜观察发现,青春期促性腺激素释放激素-增强型绿色荧光蛋白转基因大鼠视前区促性腺激素释放激素神经元呈类似倒Y形分布。青春前期促性腺激素释放激素神经元一般呈单极和双极,两种神经元都可分为棘状突起较少的光滑型和棘状突起较多的不规则型,促性腺激素释放激素神经元数量及棘状突起数量也随发育成熟而增加,但上述变化无性别差异。免疫荧光染色显示,突触素的表达靠近促性腺激素释放激素神经元远端,显示某些突触前端与促性腺激素释放激素神经元可以形成突触连接。

关键词: 神经再生, 视前区, 促性腺激素释放激素, 神经元, 青春期, 促黄体生成素, 基因转移, 双极神经元

Abstract:

Gonadotropin-releasing hormone (GnRH) neurons in the preoptic area may undergo morphological changes during the pubertal period when their activities are upregulated. To clarify the regulatory mechanism of puberty onset, this study aimed to investigate the morphological changes of GnRH neurons in the preoptic area of GnRH-enhanced green fluorescent protein transgenic rats. Under confocal laser microscopy, pubertal GnRH neurons exhibited an inverted Y distribution pattern. Prepubertal GnRH neurons were generally unipolar and bipolar, and were distinguished as smooth type cells with few small processes or irregular type cells with many spine-like processes in the proximal dendrites. The number of GnRH neurons in the preoptic area and spine-like processes were increased during the course of reproductive maturation. There was no significant difference between male and female rats. Immunofluorescence staining revealed synaptophysin punctae close to the distal end of GnRH neurons, indicating that some presynaptic terminals may form a synaptic linkage with these neurons.