中国神经再生研究(英文版) ›› 2014, Vol. 9 ›› Issue (19): 1740-1744.doi: 10.4103/1673-5374.143416

• 原著:脑损伤修复保护与再生 • 上一篇    下一篇

骨髓间充质干细胞移植可增加冷应激大鼠海马神经元的数量

  

  • 收稿日期:2014-07-20 出版日期:2014-10-15 发布日期:2014-10-15

Therapeutic effect of bone marrow mesenchymal stem cells on cold stress induced changes in the hippocampus of rats

Saravana Kumar Sampath Kumar 1, Saraswathi Perumal 2, Vijayaraghavan Rajagopalan 2   

  1. 1 Department of Anatomy, Saveetha Dental College, Chennai, India
    2 Saveetha University, Chennai, Tamil Nadu, India
  • Received:2014-07-20 Online:2014-10-15 Published:2014-10-15
  • Contact: Saravana Kumar S., Department of Anatomy, Saveetha Dental College, Chennai-77, India,dr.sharan_anatomist@yahoo.com.

摘要:

冷应激可导致海马结构异常变化,从而引发与之相关的学习记忆功能减退。实验设计每日强迫大鼠在10 ±2o C的冷水中游泳,持续1个月,建立冷应激大鼠模型。给予冷应激大鼠颈静脉注射1 × 106 骨髓间充质干细胞。结果显示,骨髓间充质干细胞移植使冷应激大鼠海马CA1区神经元数量明显增加,神经功能明显改善,说明骨髓间充质干细胞移植治疗冷应激导致的海马神经元损伤具有潜力。

关键词: 神经再生, 骨髓间充质干细胞, 冷应激, 海马, 神经元, 组织学

Abstract:

The present study aims to evaluate the effect of bone marrow mesenchymal stem cells on cold    stress induced neuronal changes in hippocampal CA1 region of Wistar rats. Bone marrow mesenchymal stem cells were isolated from a 6-week-old Wistar rat. Bone marrow from adult femora and tibia was collected and mesenchymal stem cells were cultured in minimal essential medium containing 10% heat-inactivated fetal bovine serum and were sub-cultured. Passage 3 cells were analyzed by flow cytometry for positive expression of CD44 and CD90 and negative expression of CD45. Once CD44 and CD90 positive expression was achieved, the cells were cultured again to 90% confluence for later experiments. Twenty-four rats aged 8 weeks old were randomly and evenly divided into normal control, cold water swim stress (cold stress), cold stress + PBS (intravenous infusion), and cold stress + bone marrow mesenchymal stem cells (1 × 106; intravenous infusion) groups. The total period of study was 60 days which included 1 month stress period followed by 1 month treatment. Behavioral functional test was performed during the entire study period. After treatment, rats were sacrificed for histological studies. Treatment with bone marrow mesenchymal stem cells significantly increased the number of neuronal cells in hippocampal CA1 region. Adult bone marrow mesenchymal stem cells injected by intravenous administration show potential therapeutic effects in cognitive decline associated with stress-related lesions.

Key words: nerve regeneration, bone marrow, mesenchymal stem cells, hippocampus, cold stress, intravenous, cognition, neuronal cells, CA1 region, neural regeneration