中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (7): 1166-1171.doi: 10.4103/1673-5374.156992

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

纤维蛋白胶促进早期周围神经再生过程中的轴突延长

  

  • 收稿日期:2015-04-04 出版日期:2015-07-24 发布日期:2015-07-24
  • 基金资助:

    Charité – Universitätsmedizin Berlin基金项目

Fibrin glue repair leads to enhanced axonal elongation during early peripheral nerve regeneration in an in vivo mouse model

Georgios Koulaxouzidis 1, Gernot Reim 1, Christian Witzel 2   

  1. 1 Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany
    2 Plastic and Reconstructive Surgery – Interdisciplinary Breast Center Charité – Universitätsmedizin Berlin, Germany
  • Received:2015-04-04 Online:2015-07-24 Published:2015-07-24
  • Contact: Georgios Koulaxouzidis, M.D., georgios.koulaxouzidis@uniklinik-freiburg.de
  • Supported by:

    This study was supported by funding from the Charité – Universitätsmedizin Berlin.

摘要:

纤维蛋白胶在粘合修复损伤周围神经方面的作用已逐渐得到认可,有代替周围神经损伤修复的显微外科手术的潜力,但尚无证据显示两种方法对早期周围神经再生过程中形态学改变的影响。实验以取自不带荧光小鼠的坐骨神经段1cm移植修复thy-1黄色荧光蛋白标记小鼠的坐骨神经缺损。修复方法为:一侧坐骨神经应用显微外科缝合,对侧应用纤维蛋白胶粘合。7d后结果显示,相较于显微缝合,纤维蛋白胶粘合修复的神经再生轴突向末梢神经延伸的距离更长,分叉轴突百分比更高。纤维蛋白胶粘合修复对早期周围神经再生的促进作用在某种程度上优于显微缝合。

关键词: 纤维蛋白胶, 坐骨神经损伤, 周围神经再生, thy-1-YFP小鼠, 轴突分叉, 轴突分支

Abstract:

Microsurgical suturing is the gold standard of nerve coaptation. Although literature on the usefulness of fibrin glue as an alternative is becoming increasingly available, it remains contradictory. Furthermore, no data exist on how both repair methods might influence the morphological aspects (arborization; branching) of early peripheral nerve regeneration. We used the sciatic nerve transplantation model in thy-1 yellow fluorescent protein mice (YFP; n = 10). Pieces of nerve (1cm) were grafted from YFP-negative mice (n = 10) into those expressing YFP. We performed microsuture coaptations on one side and used fibrin glue for repair on the contralateral side. Seven days after grafting, the regeneration distance, the percentage of regenerating and arborizing axons, the number of branches per axon, the coaptation failure rate, the gap size at the repair site and the time needed for surgical repair were all investigated. Fibrin glue repair resulted in regenerating axons travelling further into the distal nerve. It also increased the percentage of arborizing axons. No coaptation failure was detected. Gap sizes were comparable in both groups. Fibrin glue significantly reduced surgical repair time. The increase in regeneration distance, even after the short period of time, is in line with the results of others that showed faster axonal regeneration after fibrin glue repair. The increase in arborizing axons could be another explanation for better functional and electrophysiological results after fibrin glue repair. Fibrin glue nerve coaptation seems to be a promising alternative to microsuture repair.

Key words: nerve regeneration, fibrin glue, peripheral nerve regeneration, thy-1-YFP mice, sciatic nerve, branching, arborisation, neural regeneration