中国神经再生研究(英文版)

中国神经再生研究(英文版) ›› 2018, Vol. 13 ›› Issue (9): 1650-1656.doi: 10.4103/1673-5374.237139

• 原著:周围神经损伤修复保护与再生 • 上一篇    下一篇

柴胡皂苷a促进IL-10表达并抑制坐骨神经损伤后的瘢痕形成

  

  • 收稿日期:2018-05-22 出版日期:2018-09-15 发布日期:2018-09-15
  • 基金资助:

    国家自然科学基金(11672332，11102235，8167050417)；国家重点研究开发计划(2016YFC1101500)；天津市重点科技支持基金会(17YFZCSY 00620)；天津市自然科学基金(15JCYBJC28600，17JCZDJC 35400)

 Saikosaponin a increases interleukin-10 expression and inhibits scar formation after sciatic nerve injury

 Meng-Qiang Huang1, 2, Xiao-Yu Cao3, Xu-Yi Chen4, Ying-Fu Liu5, Shuang-Long Zhu1, 2, Zhong-Lei Sun6, Xian-Bin Kong7, Jing-Rui Huo5, Sai Zhang4, Yun-Qiang Xu1, 2   

  1. 1 Graduate School, Tianjin Medical University, Tianjin, China
    2 Department of Orthopedics, Tianjin Medical University General Hospital, Tinajin, China
    3 Department of Rehabilitation Medicine, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui Province, China
    4 Tianjin Key Laboratory of Neurotrauma Repair, Institute of Traumatic Brain Injury and Neuroscience, Center for Neurology and Neurosurgery of Affiliated Hospital, Logistics University of Chinese People’s Armed Police Force, Tianjin, China
    5 Science and Technology Experiment Center, Cangzhou Medical College, Cangzhou, Hebei Province, China
    6 Graduate School, Jinzhou Medical University, Jinzhou, Liaoning Province, China
    7 Graduate School, Tianjin University of Traditional Chinese Medicine, Tianjin, China
  • Received:2018-05-22 Online:2018-09-15 Published:2018-09-15
  • Contact: Yun-Qiang Xu, M.D., Ph.D. or Sai Zhang, M.D., Ph.D.,docxu@sina.com or zhangsai718@vip.126.com
  • Supported by:

    This work was financially supported by the National Natural Science Foundation of China, No. 11672332, 11102235, 8167050417; the National Key Research and Development Plan of China, No. 2016YFC1101500; the Key Science and Technology Support Foundation of Tianjin City of China, No. 17YFZCSY00620; the Natural Science Foundation of Tianjin City of China, No. 15JCYBJC28600, 17JCZDJC35400.

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摘要：

周围神经损伤后的神经瘢痕会严重阻碍神经的再生和功能恢复，而抗炎因子IL-10可抑制神经瘢痕的形成。柴胡皂苷a是一种提取自中国中药柴胡的单体分子，已被证明在脊髓损伤、颅脑损伤等疾病发挥抗炎作用，但在周围神经损伤中的作用未见报道。为此，实验将设计大鼠随机分为3组：sham组暴露左侧坐骨神经后直接缝合，SNI+SSa组及SNI组进行左侧坐骨神经离断缝合后连续腹腔注射7 d柴胡皂苷a(10 mg/kg剂量)或等量生理盐水，1 d/次。ELISA结果显示损伤后7 d，SNI+SSa组大鼠坐骨神经中IL-10表达量明显高于SNI组；而Masson染色和WB结果显示，损伤后8周，SNI+SSa组大鼠的I型和III型胶原蛋白表达量及神经瘢痕形成均明显少于SNI组；与此同时，坐骨神经指数和神经电生理检测结果显示，SNI+SSa组大鼠下肢坐骨神经功能指数和神经传导速度也明显好于SNI组。总结上述数据说明，柴胡皂苷a可促进大鼠坐骨神经损伤后抗炎因子IL-10的表达，并减少神经瘢痕的形成，从而促进周围神经功能恢复。

orcid:0000-0001-8790-1212(Yun-Qiang Xu)
        0000-0003-2645-0168(Sai Zhang)

关键词: 柴胡皂苷a, 抗炎因子, 炎症, 白细胞介素10, 神经瘢痕, 周围神经损伤, 坐骨神经损伤, 坐骨神经功能指数, 神经传导速度, 神经电生理功能, 神经再生

Abstract:

Nerve scarring after peripheral nerve injury can severely hamper nerve regeneration and functional recovery. Further, the anti-inflammatory cytokine, interleukin-10, can inhibit nerve scar formation. Saikosaponin a (SSa) is a monomer molecule extracted from the Chinese medicine, Bupleurum. SSa can exert anti-inflammatory effects in spinal cord injury and traumatic brain injury. However, it has not been shown whether SSa can play a role in peripheral nerve injury. In this study, rats were randomly assigned to three groups. In the sham group, the left sciatic nerve was directly sutured after exposure. In the sciatic nerve injury (SNI) + SSa and SNI groups, the left sciatic nerve was sutured and continuously injected daily with SSa (10 mg/kg) or an equivalent volume of saline for 7 days. Enzyme linked immunosorbent assay results demonstrated that at 7 days after injury, interleukin-10 level was considerably higher in the SNI + SSa group than in the SNI group. Masson staining and western blot assay demonstrated that at 8 weeks after injury, type I and III collagen content was lower and nerve scar formation was visibly less in the SNI + SSa group compared with the SNI group. Simultaneously, sciatic functional index and nerve conduction velocity were improved in the SNI + SSa group compared with the SNI group. These results confirm that SSa can increase the expression of the anti-inflammatory factor, interleukin-10, and reduce nerve scar formation to promote functional recovery of injured sciatic nerve.

Key words: nerve regeneration, saikosaponin a, anti-inflammatory factor, inflammation, interleukin-10, nerve scar, peripheral nerve injury, sciatic nerve injury, sciatic functional index, nerve conduction velocity, neuroelectrophysiological function, neural regeneration