中国神经再生研究(英文版) ›› 2015, Vol. 10 ›› Issue (6): 932-937.doi: 10.4103/1673-5374.158353

• 原著:神经损伤修复保护与再生 • 上一篇    下一篇

硫化氢对局灶性脑缺血再灌注损伤的脑保护作用

  

  • 收稿日期:2015-04-20 出版日期:2015-06-18 发布日期:2015-06-18
  • 基金资助:

    国家自然科学基金资助项目(No 81371346,81271376);新乡医学院优秀硕士资助课题;河南省教育厅科学技术研究重点项目(No 14A310019)

Hydrogen sulfide intervention in focal cerebral ischemia/reperfusion injury in rats

Xin-juan Li, Chao-kun Li, Lin-yu Wei, Na Lu, Guo-hong Wang, Hong-gang Zhao, Dong-liang Li   

  1. Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang, Henan Province, China
  • Received:2015-04-20 Online:2015-06-18 Published:2015-06-18
  • Contact: Dong-liang Li, xyldl@xxmu.edu.cn.
  • Supported by:

    This study was financially supported by grants from the National Natural Science Foundation of China, No. 81371346, 81271376; Outstanding Postgraduate Fund of Xinxiang Medical University; and Science and Technology Key Research Project of Henan Provincial Education Department of China, No. 14A310019.

摘要:

未进一步了解硫化氢对脑缺血再灌注引起的神经元损伤发挥保护作用的机制。实验通过线栓法建立大鼠大脑中动脉栓塞模型,于脑缺血后10 min腹腔注射硫氢化钠。以免疫荧光法检测显示硫氢化钠可使脑缺血再灌注组大脑皮质、海马CA1区P2X7免疫阳性细胞数、死亡率、Longa神经功能缺失评分和脑梗死体积均显著降低,结果说明硫化氢可通过下调局灶性脑缺血再灌注损伤大鼠脑组织P2X7受体的表达,发挥脑保护效应。

关键词: 神经再生, 脑损伤, 硫化氢, 脑缺血, 再灌注损伤, P2X7受体, 2, 3, 5-三苯基氯化四氮唑染色, 动物模型, 保护作用, 硫氢化钠, 免疫荧光, 大脑中动脉栓塞, 国家自然科学基金

Abstract:

The present study aimed to explore the mechanism underlying the protective effects of hydrogen sulfide against neuronal damage caused by cerebral ischemia/reperfusion. We established the middle cerebral artery occlusion model in rats via the suture method. Ten minutes after middle cerebral artery occlusion, the animals were intraperitoneally injected with hydrogen sulfide donor compound sodium hydrosulfide. Immunofluorescence revealed that the immunoreactivity of P2X7 in the cerebral cortex and hippocampal CA1 region in rats with cerebral ischemia/reperfusion injury decreased with hydrogen sulfide treatment. Furthermore, treatment of these rats with hydrogen sulfide significantly lowered mortality, the Longa neurological deficit scores, and infarct volume. These results indicate that hydrogen sulfide may be protective in rats with local cerebral ischemia/reperfusion injury by down-regulating the expression of P2X7 receptors.

Key words: nerve regeneration, brain injury, hydrogen sulfide, cerebral ischemia/reperfusion injury, P2X7 receptor, 2,3,5-triphenyl-2H-tetrazolium chloride staining, animal model, protection, sodium hydrosulfide, immunofluorescence, middle cerebral artery occlusion, NSFC grant, neural regeneration